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481. problems with dilutions: sensitivity - (reply: 13)
482. Mininum number of concentrations in a 10-fold dilution series standard... - (reply: 6)
483. How to remove gDNA from small amounts of RNA - (reply: 4)
484. quanitifying single stranded DNA using a ds standard - (reply: 1)
485. Target Amplicon Length for real-time PCR - (reply: 4)
486. Primer Optimiztion - can this be done in water (reply: 8)
487. RT PCR for larger amplicon - (reply: 2)
488. ct Validation test - using GAPDH endogenous (reply: 3)
489. increasing sensitivity and reducing primer dimmer - (reply: 11)
490. what's the difference between Ct and Cp values? - pardon my ignorance!! (reply: 3)
491. can Primers for RT-PCR be used Real-Time PCR directly? - (reply: 2)
492. GAPDH endogenous control gene - (reply: 2)
493. How excess template can inhibit the PCR reaction? - (reply: 1)
494. need advice on qPCR results (see attachment) - melting curve - gel picture (reply: 5)
495. primer selection for RT prior to real time - an insiders hint!!! (reply: 1)
496. Strange problems with B-actin amplification - (reply: 1)
497. problem with b-actin CT values - (reply: 4)
498. Diluting your cDNA for qRT-PCR? - (reply: 5)
499. Standard curve dillutions not 3 cycles apart - (reply: 2)
500. SYBR VS TAqman- What is the difference other than PRimers? - TAqman works and SYBR doesnt (reply: 1)
501. SD of individuals within groups - (reply: 1)
502. RT-PCR - not qPCR! urgent please - how to choose primers (reply: 3)
503. Time taken for RNA to denature - (reply: 2)
504. UV sterilization! - (reply: 2)
505. UV steriliser - (reply: 6)
506. dynamic well factors failed in a BIO-RAD machine - (reply: 3)
507. how do you eat yours... - (reply: 1)
508. gene of interest or housekeeping gene shift...? - (reply: 3)
509. Gel and Ct-values relationship: is there a probe problem? - Bands on gel don't fit my expectations... (reply: 2)
510. Is a 100bp PCR product more prone to give non-specific signals in a hybridisatio - (reply: 1)
482. Mininum number of concentrations in a 10-fold dilution series standard... - (reply: 6)
483. How to remove gDNA from small amounts of RNA - (reply: 4)
484. quanitifying single stranded DNA using a ds standard - (reply: 1)
485. Target Amplicon Length for real-time PCR - (reply: 4)
486. Primer Optimiztion - can this be done in water (reply: 8)
487. RT PCR for larger amplicon - (reply: 2)
488. ct Validation test - using GAPDH endogenous (reply: 3)
489. increasing sensitivity and reducing primer dimmer - (reply: 11)
490. what's the difference between Ct and Cp values? - pardon my ignorance!! (reply: 3)
491. can Primers for RT-PCR be used Real-Time PCR directly? - (reply: 2)
492. GAPDH endogenous control gene - (reply: 2)
493. How excess template can inhibit the PCR reaction? - (reply: 1)
494. need advice on qPCR results (see attachment) - melting curve - gel picture (reply: 5)
495. primer selection for RT prior to real time - an insiders hint!!! (reply: 1)
496. Strange problems with B-actin amplification - (reply: 1)
497. problem with b-actin CT values - (reply: 4)
498. Diluting your cDNA for qRT-PCR? - (reply: 5)
499. Standard curve dillutions not 3 cycles apart - (reply: 2)
500. SYBR VS TAqman- What is the difference other than PRimers? - TAqman works and SYBR doesnt (reply: 1)
501. SD of individuals within groups - (reply: 1)
502. RT-PCR - not qPCR! urgent please - how to choose primers (reply: 3)
503. Time taken for RNA to denature - (reply: 2)
504. UV sterilization! - (reply: 2)
505. UV steriliser - (reply: 6)
506. dynamic well factors failed in a BIO-RAD machine - (reply: 3)
507. how do you eat yours... - (reply: 1)
508. gene of interest or housekeeping gene shift...? - (reply: 3)
509. Gel and Ct-values relationship: is there a probe problem? - Bands on gel don't fit my expectations... (reply: 2)
510. Is a 100bp PCR product more prone to give non-specific signals in a hybridisatio - (reply: 1)