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571. What happens to PRIMERS at 72 C ? - (reply: 5)
572. No PCR product detected in agraose gel - (reply: 7)
573. 2 melting peaks in real-time PCR - (reply: 1)
574. Melting Curve giving flutating Ct values - Advice on primer design? (reply: 9)
575. What causes two peaks in melting curve? - (reply: 11)
576. Premature Fluorescence Increase - (reply: 1)
577. Minimal total volume for real time - (reply: 4)
578. qPCR with Corbett Rotor Gene 6000 - (reply: 3)
579. Does anyone have the spreadsheet for pfaffl method? - (reply: 1)
580. Relative Quantification in real-time RT-PCR - (reply: 5)
581. Wierd amplification pattern. - (reply: 8)
582. No RT control showed strange results - (reply: 3)
583. Increased Ct values, but why? - (reply: 2)
584. Two design programs give very different primer Tm values - RT-PCR (reply: 5)
585. gene expression assay - (reply: 1)
586. Inconsistent results from replicates in Taqman real-time PCR - (reply: 26)
587. need advice on efficiency - (reply: 1)
588. NTC's in RT or just qPCR? - (reply: 3)
589. Statistical test for relative quantification - (reply: 3)
590. problem in RNA extraction from mice liver and spleen - (reply: 2)
591. qPCR Efficiencies over 100% - Looking for references (reply: 1)
592. HK gene overabundance - (reply: 3)
593. Annealing vs melting Ts for real time PCR primers - (reply: 1)
594. relative quantification, primer pair problem - (reply: 1)
595. Getting Ct values in empty wells - (reply: 1)
596. weird standard curve - (reply: 5)
597. Untreated Control shows low HK status - Treatment effect? (reply: 1)
598. Stratagene Reference RNA - (reply: 1)
599. ddCt or standard curve - (reply: 4)
600. qPCR, no RT controls always positive (as cDNA samples) - (reply: 4)
572. No PCR product detected in agraose gel - (reply: 7)
573. 2 melting peaks in real-time PCR - (reply: 1)
574. Melting Curve giving flutating Ct values - Advice on primer design? (reply: 9)
575. What causes two peaks in melting curve? - (reply: 11)
576. Premature Fluorescence Increase - (reply: 1)
577. Minimal total volume for real time - (reply: 4)
578. qPCR with Corbett Rotor Gene 6000 - (reply: 3)
579. Does anyone have the spreadsheet for pfaffl method? - (reply: 1)
580. Relative Quantification in real-time RT-PCR - (reply: 5)
581. Wierd amplification pattern. - (reply: 8)
582. No RT control showed strange results - (reply: 3)
583. Increased Ct values, but why? - (reply: 2)
584. Two design programs give very different primer Tm values - RT-PCR (reply: 5)
585. gene expression assay - (reply: 1)
586. Inconsistent results from replicates in Taqman real-time PCR - (reply: 26)
587. need advice on efficiency - (reply: 1)
588. NTC's in RT or just qPCR? - (reply: 3)
589. Statistical test for relative quantification - (reply: 3)
590. problem in RNA extraction from mice liver and spleen - (reply: 2)
591. qPCR Efficiencies over 100% - Looking for references (reply: 1)
592. HK gene overabundance - (reply: 3)
593. Annealing vs melting Ts for real time PCR primers - (reply: 1)
594. relative quantification, primer pair problem - (reply: 1)
595. Getting Ct values in empty wells - (reply: 1)
596. weird standard curve - (reply: 5)
597. Untreated Control shows low HK status - Treatment effect? (reply: 1)
598. Stratagene Reference RNA - (reply: 1)
599. ddCt or standard curve - (reply: 4)
600. qPCR, no RT controls always positive (as cDNA samples) - (reply: 4)