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271. efficiency higher than 1 - (reply: 1)
272. phantom non specific amplification at high ct values - (reply: 7)
273. Does anyone know how to design LUX primers - (reply: 2)
274. quality of cDNA - (reply: 1)
275. plz guide me.i got many band in one well in agrose gel electrophoresis - PCR (reply: 8)
276. Total RNA concentration - (reply: 1)
277. How many -RT Controls do I need - (reply: 3)
278. Something about absolute quantification... - Please help me understand this!! (reply: 1)
279. quantifying my gene expression in 5 different cell lines - (reply: 1)
280. High initial fluorescence - need help with some results... (reply: 2)
281. Storage of a plate for real time PCR - Ever stored your already prepared plate for real time PCR? (reply: 7)
282. lower R square for my standard curve - (reply: 1)
283. double melt curves on rtPCR of DNA samples - (reply: 2)
284. Help: Relative Standard Curve Method - (reply: 1)
285. Primer3 results - (reply: 2)
286. TaqMan dual-labelled probe low fluorescence problems - (reply: 2)
287. comparison of mRNA expression - (reply: 1)
288. real time pcr with RNA co-precipitated with glycogen - (reply: 3)
289. urgent help-how to get amplification from very low target copy number and how to - "Taqman probe" qPCR and "rotor gene 6000" machine (reply: 4)
290. Alternative software to read .sds files? - (reply: 1)
291. slope is not even close -3.22 - (reply: 4)
292. Primer express? - Do you use it? (reply: 2)
293. What is the proper upper limit of false priming in real time? - (reply: 3)
294. PCR of mitochondrial fraction of nuclear-coded mitochondrial proteins - (reply: 4)
295. What is inhibiting my RTPCR? - Are environmental samples too complex? (reply: 6)
296. gene-specific Primer for qRT-PCR - (reply: 2)
297. BSA in PCR reaction - (reply: 1)
298. range of ratios calculated by the pfaffl method? - how to calculate them? (reply: 2)
299. Can these dissociation curves be used? - (reply: 7)
300. Has anyone seen this type of squigly signal? - RTQPCR squigly signal (reply: 3)
272. phantom non specific amplification at high ct values - (reply: 7)
273. Does anyone know how to design LUX primers - (reply: 2)
274. quality of cDNA - (reply: 1)
275. plz guide me.i got many band in one well in agrose gel electrophoresis - PCR (reply: 8)
276. Total RNA concentration - (reply: 1)
277. How many -RT Controls do I need - (reply: 3)
278. Something about absolute quantification... - Please help me understand this!! (reply: 1)
279. quantifying my gene expression in 5 different cell lines - (reply: 1)
280. High initial fluorescence - need help with some results... (reply: 2)
281. Storage of a plate for real time PCR - Ever stored your already prepared plate for real time PCR? (reply: 7)
282. lower R square for my standard curve - (reply: 1)
283. double melt curves on rtPCR of DNA samples - (reply: 2)
284. Help: Relative Standard Curve Method - (reply: 1)
285. Primer3 results - (reply: 2)
286. TaqMan dual-labelled probe low fluorescence problems - (reply: 2)
287. comparison of mRNA expression - (reply: 1)
288. real time pcr with RNA co-precipitated with glycogen - (reply: 3)
289. urgent help-how to get amplification from very low target copy number and how to - "Taqman probe" qPCR and "rotor gene 6000" machine (reply: 4)
290. Alternative software to read .sds files? - (reply: 1)
291. slope is not even close -3.22 - (reply: 4)
292. Primer express? - Do you use it? (reply: 2)
293. What is the proper upper limit of false priming in real time? - (reply: 3)
294. PCR of mitochondrial fraction of nuclear-coded mitochondrial proteins - (reply: 4)
295. What is inhibiting my RTPCR? - Are environmental samples too complex? (reply: 6)
296. gene-specific Primer for qRT-PCR - (reply: 2)
297. BSA in PCR reaction - (reply: 1)
298. range of ratios calculated by the pfaffl method? - how to calculate them? (reply: 2)
299. Can these dissociation curves be used? - (reply: 7)
300. Has anyone seen this type of squigly signal? - RTQPCR squigly signal (reply: 3)