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211. TaqMan - 18S curve not sigmoidal, replicates are different - (reply: 4)
212. Taqman PCR master mix - (reply: 4)
213. Primer design (long transcripts) - (reply: 2)
214. Primers for Real time PCR - Really need some advice (reply: 1)
215. Standard curve needed? - (reply: 1)
216. Cell lysis buffer for real time RT-PCR - Request for cell lysis buffer recipe or relevant scientific paper (reply: 1)
217. 18S trouble in cleared MMG - (reply: 2)
218. RNA amplification - (reply: 3)
219. Problems with endogenous control - S18 (reply: 2)
220. pfaffl method - (reply: 2)
221. analyzing from multiple plates - (reply: 10)
222. Storage: cDNA or mRNA - (reply: 3)
223. help!real-time PCR to see gene's expression after treatment with cytokin - (reply: 4)
224. SYbrgreen PCR protocol for the rookie - (reply: 1)
225. Syber green - length (reply: 1)
226. absolute qPCR problem - (reply: 1)
227. Silly question - primer design (reply: 1)
228. Setting up and analysing real time PCR - (reply: 2)
229. Simple QPCR question? - (reply: 1)
230. Reducing temperature-dependence of probe? - (reply: 2)
231. SYBR green PCR - (reply: 4)
232. How does everyone dispose of pipette tips in a clean hood? - (reply: 6)
233. Melting curve - SYBR Green PCR (reply: 8)
234. Taqman Vs SYBR Green - new to qRT-PCR and trying to learn.... (reply: 1)
235. How to ressuspend primers for Sybr? - (reply: 3)
236. Basic question on collecting liver for RT-PCR - Never did this before, won't get to do this again with these anima (reply: 3)
237. mRNA quantitation - need some suggestions (reply: 1)
238. Error bars in Multi-plate analysis - (reply: 2)
239. SYBR green ready mixes - need opinions please (reply: 3)
240. Q-PCR for mycoplasmas - performance is way to low (reply: 2)
212. Taqman PCR master mix - (reply: 4)
213. Primer design (long transcripts) - (reply: 2)
214. Primers for Real time PCR - Really need some advice (reply: 1)
215. Standard curve needed? - (reply: 1)
216. Cell lysis buffer for real time RT-PCR - Request for cell lysis buffer recipe or relevant scientific paper (reply: 1)
217. 18S trouble in cleared MMG - (reply: 2)
218. RNA amplification - (reply: 3)
219. Problems with endogenous control - S18 (reply: 2)
220. pfaffl method - (reply: 2)
221. analyzing from multiple plates - (reply: 10)
222. Storage: cDNA or mRNA - (reply: 3)
223. help!real-time PCR to see gene's expression after treatment with cytokin - (reply: 4)
224. SYbrgreen PCR protocol for the rookie - (reply: 1)
225. Syber green - length (reply: 1)
226. absolute qPCR problem - (reply: 1)
227. Silly question - primer design (reply: 1)
228. Setting up and analysing real time PCR - (reply: 2)
229. Simple QPCR question? - (reply: 1)
230. Reducing temperature-dependence of probe? - (reply: 2)
231. SYBR green PCR - (reply: 4)
232. How does everyone dispose of pipette tips in a clean hood? - (reply: 6)
233. Melting curve - SYBR Green PCR (reply: 8)
234. Taqman Vs SYBR Green - new to qRT-PCR and trying to learn.... (reply: 1)
235. How to ressuspend primers for Sybr? - (reply: 3)
236. Basic question on collecting liver for RT-PCR - Never did this before, won't get to do this again with these anima (reply: 3)
237. mRNA quantitation - need some suggestions (reply: 1)
238. Error bars in Multi-plate analysis - (reply: 2)
239. SYBR green ready mixes - need opinions please (reply: 3)
240. Q-PCR for mycoplasmas - performance is way to low (reply: 2)