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391. inconsistent result from real time PCR - (reply: 2)
392. Reverse-transcription - how much RNA will be converted into cDNA (reply: 4)
393. different Ct ... - (reply: 2)
394. Homemade TaqMan PCR Mastermix? - (reply: 1)
395. mRNA expression too low? - (reply: 3)
396. Getting signal in the negative controls - (reply: 5)
397. qPCR using random primers - (reply: 4)
398. no bands in PCR reaction analysis - does centrifuge time matter? (reply: 2)
399. Determining low product cut off using Ct - How to decide Ct level at which expression is unreliable (reply: 1)
400. Verification of real-time amplification specificity - (reply: 1)
401. 1. ABI Power SYBR® Green primer efficiency? - (reply: 1)
402. DNA Standard - DNA Standard for Calibration of spectrophotometer (reply: 2)
403. Interpretation of comparative quantitation result - (reply: 3)
404. Is this contamination? - (reply: 1)
405. standard curve - normalization - (reply: 1)
406. Real time - (reply: 2)
407. 18s control higher then suppose to be - (reply: 2)
408. Problems with RNA extraction or QPCR for AI - (reply: 2)
409. Calculating copy number for standard curve - (reply: 1)
410. Primer Efficiency (how to change the slope) - (reply: 5)
411. To compare normal and cancer tissue - (reply: 5)
412. My control is a group of several subjects - How to perform the analysis? (reply: 1)
413. Taqman probe Tm ASAP! - (reply: 1)
414. Inhibitory component of a PCR assay - (reply: 10)
415. contamination in cDNA - (reply: 4)
416. Ct value of replicates travel very far - (reply: 7)
417. No-RT control in qRT-PCR - (reply: 5)
418. checking quality of DNA isolated - (reply: 1)
419. RT PCR - NO PCR PRODUCT (reply: 3)
420. about cDNA - (reply: 1)
392. Reverse-transcription - how much RNA will be converted into cDNA (reply: 4)
393. different Ct ... - (reply: 2)
394. Homemade TaqMan PCR Mastermix? - (reply: 1)
395. mRNA expression too low? - (reply: 3)
396. Getting signal in the negative controls - (reply: 5)
397. qPCR using random primers - (reply: 4)
398. no bands in PCR reaction analysis - does centrifuge time matter? (reply: 2)
399. Determining low product cut off using Ct - How to decide Ct level at which expression is unreliable (reply: 1)
400. Verification of real-time amplification specificity - (reply: 1)
401. 1. ABI Power SYBR® Green primer efficiency? - (reply: 1)
402. DNA Standard - DNA Standard for Calibration of spectrophotometer (reply: 2)
403. Interpretation of comparative quantitation result - (reply: 3)
404. Is this contamination? - (reply: 1)
405. standard curve - normalization - (reply: 1)
406. Real time - (reply: 2)
407. 18s control higher then suppose to be - (reply: 2)
408. Problems with RNA extraction or QPCR for AI - (reply: 2)
409. Calculating copy number for standard curve - (reply: 1)
410. Primer Efficiency (how to change the slope) - (reply: 5)
411. To compare normal and cancer tissue - (reply: 5)
412. My control is a group of several subjects - How to perform the analysis? (reply: 1)
413. Taqman probe Tm ASAP! - (reply: 1)
414. Inhibitory component of a PCR assay - (reply: 10)
415. contamination in cDNA - (reply: 4)
416. Ct value of replicates travel very far - (reply: 7)
417. No-RT control in qRT-PCR - (reply: 5)
418. checking quality of DNA isolated - (reply: 1)
419. RT PCR - NO PCR PRODUCT (reply: 3)
420. about cDNA - (reply: 1)