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421. RNA quantification for Real Time RT-PCR - (reply: 2)
422. first time doing quantitative real time PCR - (reply: 1)
423. Non linear regression and sigmoidal curves to calculate quantitative RT-PCR - New paper in Biotechniques (reply: 1)
424. Problems with dissociation curves. - I know this is not new...but its still confusing (reply: 2)
425. Does non-digested RNA in cDNA sample have an effect on RT-PCR quantification? - (reply: 2)
426. Product with different Tm among samples - Sybr Green gives products with differing Tm of ~2-3 degrees (reply: 7)
427. Real time PCR of low expressing genes - Late tumor Ct (reply: 1)
428. RNA expression calculations - (reply: 1)
429. what does the slope and efficiency tell us - (reply: 1)
430. Longest Amplicon Using Real-Time PCR? - (reply: 1)
431. transgene ct comparison - help (reply: 1)
432. Ct for housekeeping gene is higher than Ct of target gene. - (reply: 1)
433. PCR efficiency - (reply: 1)
434. multiplex RT-PCR - (reply: 6)
435. cDNA for real-time PCR - (reply: 3)
436. Inconsistancy in the CT values for the same samples! - (reply: 8)
437. Genexpression analses in two different species - how calculate the efficiency (reply: 1)
438. gene expression study - between treated and untreated cells (reply: 2)
439. Mystery PCR product at lower dilutions - (reply: 3)
440. cDNA for real time PCR - for gene expression study (reply: 1)
441. PCR - qPCR inconsistent - To explain differences between normal PCR and qPCR (reply: 6)
442. normalization for real-time PCR - (reply: 4)
443. primer for real-time and reverse transcriptase PCR - (reply: 1)
444. calculate relative expression using 3 biological replicates - (reply: 1)
445. Statistical analysis of quantitative real-time PCR (qRTPCR) - Statistics on qRTPCR data (reply: 2)
446. real time contamination - (reply: 2)
447. troubles with efficiency in Taq-Man - (reply: 2)
448. Representing RT-PCR Data - (reply: 2)
449. Sybergreen vs taqman? - Comparable? (reply: 1)
450. what are some general guidelines for manipulating Taq? - (reply: 2)
422. first time doing quantitative real time PCR - (reply: 1)
423. Non linear regression and sigmoidal curves to calculate quantitative RT-PCR - New paper in Biotechniques (reply: 1)
424. Problems with dissociation curves. - I know this is not new...but its still confusing (reply: 2)
425. Does non-digested RNA in cDNA sample have an effect on RT-PCR quantification? - (reply: 2)
426. Product with different Tm among samples - Sybr Green gives products with differing Tm of ~2-3 degrees (reply: 7)
427. Real time PCR of low expressing genes - Late tumor Ct (reply: 1)
428. RNA expression calculations - (reply: 1)
429. what does the slope and efficiency tell us - (reply: 1)
430. Longest Amplicon Using Real-Time PCR? - (reply: 1)
431. transgene ct comparison - help (reply: 1)
432. Ct for housekeeping gene is higher than Ct of target gene. - (reply: 1)
433. PCR efficiency - (reply: 1)
434. multiplex RT-PCR - (reply: 6)
435. cDNA for real-time PCR - (reply: 3)
436. Inconsistancy in the CT values for the same samples! - (reply: 8)
437. Genexpression analses in two different species - how calculate the efficiency (reply: 1)
438. gene expression study - between treated and untreated cells (reply: 2)
439. Mystery PCR product at lower dilutions - (reply: 3)
440. cDNA for real time PCR - for gene expression study (reply: 1)
441. PCR - qPCR inconsistent - To explain differences between normal PCR and qPCR (reply: 6)
442. normalization for real-time PCR - (reply: 4)
443. primer for real-time and reverse transcriptase PCR - (reply: 1)
444. calculate relative expression using 3 biological replicates - (reply: 1)
445. Statistical analysis of quantitative real-time PCR (qRTPCR) - Statistics on qRTPCR data (reply: 2)
446. real time contamination - (reply: 2)
447. troubles with efficiency in Taq-Man - (reply: 2)
448. Representing RT-PCR Data - (reply: 2)
449. Sybergreen vs taqman? - Comparable? (reply: 1)
450. what are some general guidelines for manipulating Taq? - (reply: 2)