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601. Do I have to make pcr master mix fresh every time - (reply: 1)
602. 2nd round of amplification issues - (reply: 1)
603. qPCR Standard control - plasmid quantify (reply: 3)
604. What is the best positive expression control for your experiments? - Which is better? (reply: 6)
605. Plate setup- have I covered everything? - help- qRT-PCR newbie in state of confusion! (reply: 2)
606. how to choose best annealing temp. for primers? - (reply: 6)
607. how long should qPCR product be? - 150~250bp? (reply: 2)
608. Primer Dimer problem- Different perspective - COuld there be a treatment effect (reply: 4)
609. Ct difference - (reply: 1)
610. Series dilution of RNA or cDNA for qPCR standard curve? - (reply: 1)
611. Calculating relative efficiency of genes - Standard curve (reply: 4)
612. Real Time: One Melting Curve 2 gel bands - (reply: 6)
613. real time PCR machine choice - (reply: 1)
614. QPCR, where to start? - optimizing primer and template (reply: 5)
615. Another name of IkappaBalpha? - (reply: 2)
616. Taqman QPCR help - amplification of FAM right from the 0 cycle (reply: 5)
617. TaqMan probes vs Molecular Beacon probes - (reply: 1)
618. QPCR Copy number - (reply: 2)
619. designing primers for Real Time - (reply: 1)
620. Efficiency differences between target and housekeeping gene - (reply: 1)
621. RT-qPCR of bacteria mRNA from sludge - RT-PCR, Real-time PCR, mRNA, sludge (reply: 3)
622. Real time PCR late amplification - (reply: 6)
623. pulling cDNA - a big error? - (reply: 6)
624. 4 deg Celsius after PCR - (reply: 1)
625. Taqman versus SYBR Green - (reply: 2)
626. Real time machines? - (reply: 5)
627. snp analysis - choosing snps for study (reply: 1)
628. What to choose as a calibrator? - When looking at basal gene expression (reply: 7)
629. Standard Curve Issues with ABI qPCR - Problems getting good R^2 values and efficiency (reply: 2)
630. ASAP: ONE STEP Real time RT PCR kit for TWO step RT-PCR method? - (reply: 1)
602. 2nd round of amplification issues - (reply: 1)
603. qPCR Standard control - plasmid quantify (reply: 3)
604. What is the best positive expression control for your experiments? - Which is better? (reply: 6)
605. Plate setup- have I covered everything? - help- qRT-PCR newbie in state of confusion! (reply: 2)
606. how to choose best annealing temp. for primers? - (reply: 6)
607. how long should qPCR product be? - 150~250bp? (reply: 2)
608. Primer Dimer problem- Different perspective - COuld there be a treatment effect (reply: 4)
609. Ct difference - (reply: 1)
610. Series dilution of RNA or cDNA for qPCR standard curve? - (reply: 1)
611. Calculating relative efficiency of genes - Standard curve (reply: 4)
612. Real Time: One Melting Curve 2 gel bands - (reply: 6)
613. real time PCR machine choice - (reply: 1)
614. QPCR, where to start? - optimizing primer and template (reply: 5)
615. Another name of IkappaBalpha? - (reply: 2)
616. Taqman QPCR help - amplification of FAM right from the 0 cycle (reply: 5)
617. TaqMan probes vs Molecular Beacon probes - (reply: 1)
618. QPCR Copy number - (reply: 2)
619. designing primers for Real Time - (reply: 1)
620. Efficiency differences between target and housekeeping gene - (reply: 1)
621. RT-qPCR of bacteria mRNA from sludge - RT-PCR, Real-time PCR, mRNA, sludge (reply: 3)
622. Real time PCR late amplification - (reply: 6)
623. pulling cDNA - a big error? - (reply: 6)
624. 4 deg Celsius after PCR - (reply: 1)
625. Taqman versus SYBR Green - (reply: 2)
626. Real time machines? - (reply: 5)
627. snp analysis - choosing snps for study (reply: 1)
628. What to choose as a calibrator? - When looking at basal gene expression (reply: 7)
629. Standard Curve Issues with ABI qPCR - Problems getting good R^2 values and efficiency (reply: 2)
630. ASAP: ONE STEP Real time RT PCR kit for TWO step RT-PCR method? - (reply: 1)