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301. how to get rid of the DNA within RNA extraction - (reply: 3)
302. No signal in Real-time but in normal PCR?! - (reply: 2)
303. gDNA contamination in realtime PCR - (reply: 4)
304. need help to evaluate primer sets for SYBR green qPCR - (reply: 2)
305. Real-time PCR low efficiency - (reply: 1)
306. qPCR databases - (reply: 1)
307. total RNA isolation from whole blood - (reply: 7)
308. problem: low efficiency - (reply: 2)
309. QPcr for alternatively spliced products - (reply: 4)
310. qPCR primer efficiancy - (reply: 2)
311. New to Real Time, please help - (reply: 3)
312. Primer Express by ABI - (reply: 2)
313. real time PCR template - template concentration (reply: 3)
314. High Ct Values, low amplification - (reply: 3)
315. How can I get rid of the DNA contamination when prepare my total RNA sampls for - I used the RNAqueous-4PCR (isolation of DNA free RNA kit)kit,still hav (reply: 8)
316. PCR- DNA and Tm - (reply: 1)
317. Let's talk about housekeeping genes - Specifically, in Bacteria (reply: 7)
318. optimisation of reaction - extra peak in melting curve - (reply: 6)
319. cDNA synthesis and statistical variation - (reply: 1)
320. Touchdown conditions for qPCR - (reply: 1)
321. RNA extraction from leukocytes - (reply: 2)
322. Can I use different amount of cDNA template to run real time PCR? - when the amount of templates are different,Can I use reference gene to (reply: 2)
323. QPCR Quantitation of copy number - (reply: 1)
324. qrt pcr - quantitative analysis (reply: 10)
325. Detection of 10 plasmid copies - detection limit (reply: 5)
326. Allelic Discrimination - (reply: 1)
327. 10 fold dilutions show no RT-PCR product whatsoever - (reply: 1)
328. how to compare 2 different gene expression level in the same sample? - can I use ratio(A/B)=2^(CtB-CtA)to calculate these two genes' expr (reply: 5)
329. Interpretation of Output - What is a "true or real" difference? (reply: 1)
330. RNA handling for RT-PCR? - (reply: 4)
302. No signal in Real-time but in normal PCR?! - (reply: 2)
303. gDNA contamination in realtime PCR - (reply: 4)
304. need help to evaluate primer sets for SYBR green qPCR - (reply: 2)
305. Real-time PCR low efficiency - (reply: 1)
306. qPCR databases - (reply: 1)
307. total RNA isolation from whole blood - (reply: 7)
308. problem: low efficiency - (reply: 2)
309. QPcr for alternatively spliced products - (reply: 4)
310. qPCR primer efficiancy - (reply: 2)
311. New to Real Time, please help - (reply: 3)
312. Primer Express by ABI - (reply: 2)
313. real time PCR template - template concentration (reply: 3)
314. High Ct Values, low amplification - (reply: 3)
315. How can I get rid of the DNA contamination when prepare my total RNA sampls for - I used the RNAqueous-4PCR (isolation of DNA free RNA kit)kit,still hav (reply: 8)
316. PCR- DNA and Tm - (reply: 1)
317. Let's talk about housekeeping genes - Specifically, in Bacteria (reply: 7)
318. optimisation of reaction - extra peak in melting curve - (reply: 6)
319. cDNA synthesis and statistical variation - (reply: 1)
320. Touchdown conditions for qPCR - (reply: 1)
321. RNA extraction from leukocytes - (reply: 2)
322. Can I use different amount of cDNA template to run real time PCR? - when the amount of templates are different,Can I use reference gene to (reply: 2)
323. QPCR Quantitation of copy number - (reply: 1)
324. qrt pcr - quantitative analysis (reply: 10)
325. Detection of 10 plasmid copies - detection limit (reply: 5)
326. Allelic Discrimination - (reply: 1)
327. 10 fold dilutions show no RT-PCR product whatsoever - (reply: 1)
328. how to compare 2 different gene expression level in the same sample? - can I use ratio(A/B)=2^(CtB-CtA)to calculate these two genes' expr (reply: 5)
329. Interpretation of Output - What is a "true or real" difference? (reply: 1)
330. RNA handling for RT-PCR? - (reply: 4)