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61. Strange amplification pattern in DNA real time PCR - (reply: 4)
62. Difference between semi-q and qPCR - (reply: 2)
63. Multichannel Pipette for qPCR plates - Effective manual pipetting of qPCR plates (reply: 1)
64. Housekeeping genes validation - (reply: 3)
65. house keeping gene not intron spanning and sample DNase UNtreated - (reply: 1)
66. Different efficiency in different samples but the same primer - (reply: 1)
67. Ghost contamination in Real Time PCR - (reply: 4)
68. samples come at same Ct as NTC - contamination impossible (reply: 1)
69. Increasing the dNTPs in a Multiplex Rxn - (reply: 2)
70. Magnetic beads in a QPCR rxn - (reply: 1)
71. Effect of amplicon length - Effects of amplicon length-qpcr (reply: 10)
72. qPCR internal control - (reply: 3)
73. Do I need sterile RT-PCR tubes? - Real time PCR (reply: 5)
74. How to convert the Ct values to copy number - (reply: 2)
75. A quick question about series dilution - (reply: 1)
76. efficiency by std curve v/s sample-by-sample efficiency - for Corbett 3000 users (reply: 5)
77. Improving melt curves - (reply: 5)
78. Designing the primers for qRT PCR - Query - (reply: 14)
79. unsolved qPCR problem! - (reply: 1)
80. doubts about melting curve, Ct... - understanding results from 1st real time PCR (reply: 5)
81. Artifacts in early cycles - QPCR Troubleshooting (reply: 1)
82. Rotor-Gene 6000 - QPCR Machines (reply: 4)
83. taq Gold and rox buffer - (reply: 2)
84. spiking cDNA or PCR product for standanrd curve dilutions - (reply: 1)
85. how to select a calibrator for relative gene expression in real-time PCR - also about the inconsistant about the triplicate of real-time PCR (reply: 6)
86. SYBR green - (reply: 1)
87. Two peaks in melt curve when I increase master mix volume - (reply: 4)
88. contamination issue in -RT control - (reply: 1)
89. QPCR - (reply: 9)
90. QPCR - (reply: 2)
62. Difference between semi-q and qPCR - (reply: 2)
63. Multichannel Pipette for qPCR plates - Effective manual pipetting of qPCR plates (reply: 1)
64. Housekeeping genes validation - (reply: 3)
65. house keeping gene not intron spanning and sample DNase UNtreated - (reply: 1)
66. Different efficiency in different samples but the same primer - (reply: 1)
67. Ghost contamination in Real Time PCR - (reply: 4)
68. samples come at same Ct as NTC - contamination impossible (reply: 1)
69. Increasing the dNTPs in a Multiplex Rxn - (reply: 2)
70. Magnetic beads in a QPCR rxn - (reply: 1)
71. Effect of amplicon length - Effects of amplicon length-qpcr (reply: 10)
72. qPCR internal control - (reply: 3)
73. Do I need sterile RT-PCR tubes? - Real time PCR (reply: 5)
74. How to convert the Ct values to copy number - (reply: 2)
75. A quick question about series dilution - (reply: 1)
76. efficiency by std curve v/s sample-by-sample efficiency - for Corbett 3000 users (reply: 5)
77. Improving melt curves - (reply: 5)
78. Designing the primers for qRT PCR - Query - (reply: 14)
79. unsolved qPCR problem! - (reply: 1)
80. doubts about melting curve, Ct... - understanding results from 1st real time PCR (reply: 5)
81. Artifacts in early cycles - QPCR Troubleshooting (reply: 1)
82. Rotor-Gene 6000 - QPCR Machines (reply: 4)
83. taq Gold and rox buffer - (reply: 2)
84. spiking cDNA or PCR product for standanrd curve dilutions - (reply: 1)
85. how to select a calibrator for relative gene expression in real-time PCR - also about the inconsistant about the triplicate of real-time PCR (reply: 6)
86. SYBR green - (reply: 1)
87. Two peaks in melt curve when I increase master mix volume - (reply: 4)
88. contamination issue in -RT control - (reply: 1)
89. QPCR - (reply: 9)
90. QPCR - (reply: 2)