RNA extraction for real time PCR from specific brain region - (Oct/10/2008 )

Dear all,

I want to extract RNA from mouse brain tissue, (not the difficult part ) to do real time experiments on it.
The problem is that I want the RNA comming from a specific brain region and not the entire brain (this gives a completly different result)

How do I best extract this tissuepiece and preserve the RNA in it?

brains after removal and flushing the blood are immediatly immersed in liquid nitrogen to instant freez them.


Suggestions??

Dear all,

I want to extract RNA from mouse brain tissue, (not the difficult part ) to do real time experiments on it.
The problem is that I want the RNA comming from a specific brain region and not the entire brain (this gives a completly different result)

How do I best extract this tissuepiece and preserve the RNA in it?

brains after removal and flushing the blood are immediatly immersed in liquid nitrogen to instant freez them.


Suggestions??