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151. What Ct value is accepted as expression? - qRT-PCR Ct values (reply: 3)
152. recommended primers for caspase 8 & 9 - human (reply: 4)
153. qRT-PCR first timer, controls? - (reply: 9)
154. 384 well plate - do u have ever used?? (reply: 3)
155. Etbr Disposal - (reply: 2)
156. Inconsistant curves - (reply: 3)
157. probe fluorescence curve nose-dives after peak - (rather than plateau-ing: image attached) (reply: 1)
158. PAGE with Realtime PCR product (Sybr Green) - Post-staining with ethidium bromide? (reply: 4)
159. making aliquote - i am confused it will affect my result (reply: 3)
160. melting curve - (reply: 3)
161. better primer for cDNA synthesis in real time pcr - (reply: 1)
162. two peaks in the melting curves, but only one product - (reply: 6)
163. Q-PCR in microarray data validation - the consistency of the two kinds of data (reply: 2)
164. Fluorescence level in real-time - (reply: 2)
165. Should I linearise my Ct data before calulation of CVexp? - (reply: 1)
166. can you judge a 22bp primer based on the 3' 10bp? - (reply: 6)
167. Question concerning quality RNA - (reply: 1)
168. Getting primer dimers in RT-PCR on and off? - (reply: 2)
169. processing data from triplicate runs - (reply: 2)
170. PCR reaction - quick question! - (reply: 2)
171. concentrating RNA - (reply: 9)
172. light cycler cycle times - (reply: 2)
173. Weekly Decontamination Routine? - To prevent DNA contamination in a PCR lab (reply: 1)
174. PCR - PCR CONTROLS AND HOW TO DETECT POLYMORPHISM (reply: 5)
175. 1:10 serial dilution in rt-pcr with slope of -0.58 ... - (reply: 1)
176. Recipe for Impron II buffer (Promega) - We have excess enzyme and not enough buffer! (can't belive it myself) (reply: 2)
177. plate set up - (reply: 3)
178. standard PCR efficiency - No real time! (reply: 2)
179. Taqman target gene and sybr green endogenous control? - (reply: 1)
180. Could SYBR GREEN I see 60bp dsDNA? - (reply: 8)
152. recommended primers for caspase 8 & 9 - human (reply: 4)
153. qRT-PCR first timer, controls? - (reply: 9)
154. 384 well plate - do u have ever used?? (reply: 3)
155. Etbr Disposal - (reply: 2)
156. Inconsistant curves - (reply: 3)
157. probe fluorescence curve nose-dives after peak - (rather than plateau-ing: image attached) (reply: 1)
158. PAGE with Realtime PCR product (Sybr Green) - Post-staining with ethidium bromide? (reply: 4)
159. making aliquote - i am confused it will affect my result (reply: 3)
160. melting curve - (reply: 3)
161. better primer for cDNA synthesis in real time pcr - (reply: 1)
162. two peaks in the melting curves, but only one product - (reply: 6)
163. Q-PCR in microarray data validation - the consistency of the two kinds of data (reply: 2)
164. Fluorescence level in real-time - (reply: 2)
165. Should I linearise my Ct data before calulation of CVexp? - (reply: 1)
166. can you judge a 22bp primer based on the 3' 10bp? - (reply: 6)
167. Question concerning quality RNA - (reply: 1)
168. Getting primer dimers in RT-PCR on and off? - (reply: 2)
169. processing data from triplicate runs - (reply: 2)
170. PCR reaction - quick question! - (reply: 2)
171. concentrating RNA - (reply: 9)
172. light cycler cycle times - (reply: 2)
173. Weekly Decontamination Routine? - To prevent DNA contamination in a PCR lab (reply: 1)
174. PCR - PCR CONTROLS AND HOW TO DETECT POLYMORPHISM (reply: 5)
175. 1:10 serial dilution in rt-pcr with slope of -0.58 ... - (reply: 1)
176. Recipe for Impron II buffer (Promega) - We have excess enzyme and not enough buffer! (can't belive it myself) (reply: 2)
177. plate set up - (reply: 3)
178. standard PCR efficiency - No real time! (reply: 2)
179. Taqman target gene and sybr green endogenous control? - (reply: 1)
180. Could SYBR GREEN I see 60bp dsDNA? - (reply: 8)