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331. Universal probe library probes.... - (reply: 12)
332. taqman is giving initially raised then fall off amplification curve why? - changed template concentraion n baseline too.....but ? (reply: 2)
333. GAPDH primer sequences for mRNA level in HeLa cells - (reply: 2)
334. RT PCR Primer dimer tissue dependent SYBR - (reply: 1)
335. Using sybr green and Taqman probes on the same plate? - (reply: 1)
336. What different of TaqMan probe and Molecular Beacon probe ? - Why a reporter of TaqMan will be cleave, but not for a reporter of Mol (reply: 2)
337. standart curve and efficiency - (reply: 1)
338. genomic DNA for qPCR quantification - target sequence? - (reply: 1)
339. normality distribution in data - real-time PCR (reply: 2)
340. looking for a website for a newbie to learn qPCR - (reply: 3)
341. Stats on Delta Delta Ct Method, Large Std Dev - (reply: 3)
342. primer design for Syber Green - (reply: 1)
343. standard curve preparation - (reply: 1)
344. housekeeping or not housekeeping? - (reply: 3)
345. PCR efficiencies calculation - (reply: 2)
346. How do I calculate efficiency, and what does it mean? - What value would correspond to perfect efficiency? (reply: 8)
347. Reference Gene: human leukocytes - (reply: 2)
348. any problems with RT-PCR for large amplicon sizes? - very strange amplification curves (reply: 2)
349. preference for region of primer design for qRT-PCR - (reply: 1)
350. Maximum acceptable variation of Housekeeping gene - (reply: 1)
351. qPCR housekeepers - primers or probes? - (reply: 1)
352. what kind of quantification should I use? - (reply: 1)
353. which standards... - (reply: 1)
354. QPCR Oligo Database of Pathogens - (reply: 1)
355. How do I know if my cDNA are of good quality? - (reply: 7)
356. One MGB probes works, but the other does not. - (reply: 1)
357. What is happening to my 18S? - (reply: 4)
358. Extra longer unspecific product of PCR - PCR (reply: 1)
359. What is the Ct standard deviation for the same sample in the whole pcr plate? - (reply: 2)
360. real time pcr in a day - (reply: 5)
332. taqman is giving initially raised then fall off amplification curve why? - changed template concentraion n baseline too.....but ? (reply: 2)
333. GAPDH primer sequences for mRNA level in HeLa cells - (reply: 2)
334. RT PCR Primer dimer tissue dependent SYBR - (reply: 1)
335. Using sybr green and Taqman probes on the same plate? - (reply: 1)
336. What different of TaqMan probe and Molecular Beacon probe ? - Why a reporter of TaqMan will be cleave, but not for a reporter of Mol (reply: 2)
337. standart curve and efficiency - (reply: 1)
338. genomic DNA for qPCR quantification - target sequence? - (reply: 1)
339. normality distribution in data - real-time PCR (reply: 2)
340. looking for a website for a newbie to learn qPCR - (reply: 3)
341. Stats on Delta Delta Ct Method, Large Std Dev - (reply: 3)
342. primer design for Syber Green - (reply: 1)
343. standard curve preparation - (reply: 1)
344. housekeeping or not housekeeping? - (reply: 3)
345. PCR efficiencies calculation - (reply: 2)
346. How do I calculate efficiency, and what does it mean? - What value would correspond to perfect efficiency? (reply: 8)
347. Reference Gene: human leukocytes - (reply: 2)
348. any problems with RT-PCR for large amplicon sizes? - very strange amplification curves (reply: 2)
349. preference for region of primer design for qRT-PCR - (reply: 1)
350. Maximum acceptable variation of Housekeeping gene - (reply: 1)
351. qPCR housekeepers - primers or probes? - (reply: 1)
352. what kind of quantification should I use? - (reply: 1)
353. which standards... - (reply: 1)
354. QPCR Oligo Database of Pathogens - (reply: 1)
355. How do I know if my cDNA are of good quality? - (reply: 7)
356. One MGB probes works, but the other does not. - (reply: 1)
357. What is happening to my 18S? - (reply: 4)
358. Extra longer unspecific product of PCR - PCR (reply: 1)
359. What is the Ct standard deviation for the same sample in the whole pcr plate? - (reply: 2)
360. real time pcr in a day - (reply: 5)