Why not just two points on a standard curve? - (Oct/24/2008 )

Yes, I was just wondering.
If I want to have the most accurate estimation of reaction efficiency.
If I already know that I can do a nice standard curve using dilutions of (whatever) 10e1, 10e2, 10e3, 10e4, 10e5. All points lie on line.

I can spare 15 wells for standards.
I could run each dilution in triplicate.
Or I could do 8 of the 10e1 dilution and 7 of the 10e5 dilution.

What would give the best estimation?
I've noticed that the LightCycler 480 doesn't allow the construction of a standard curve unless you have more than two dilutions.

Cheers
TK

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Or, you could do the 10e1 by itself and draw the line through origin. Not.
I'd do the standard curve in triplicate, and samples in duplicates or triplicates.

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