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91. Syber green - (reply: 4)
92. Standard curve from plasmid - for cDNA quantification (reply: 6)
93. High baseline/noise probes - (reply: 2)
94. problems cleaning up small DNA products - (reply: 2)
95. interpretation of results of relative qRTPCR - please help (reply: 5)
96. New to qPCR - (reply: 2)
97. RNA from paraffin - (reply: 1)
98. Abgene PCR master mix?-MgCl concentration - (reply: 1)
99. Sybr Green real time pcr limit of detection - (reply: 1)
100. real-time PCR problem - (reply: 1)
101. Can i use old standard curve to determine gene concentration? - (reply: 1)
102. Inconsistent Real Time results- RT efficiency, high Ct values - (reply: 3)
103. Creating standard curve - (reply: 1)
104. Real-time PCR for mRNA stability - (reply: 2)
105. Cre genotyping - smeared results (reply: 1)
106. Standard Curve method RT PCR- - (reply: 2)
107. Troubleshooting in quantifying Genomic/WGA DNA - (reply: 5)
108. Large smear in PCR - (reply: 2)
109. kras mutation. best method for detection - (reply: 4)
110. developing real time PCR - (reply: 5)
111. First time Real TIme - (reply: 2)
112. qPCR - running taqman for endogenous control and SYBR for the gene - (reply: 2)
113. geNorm and normalising factor - (reply: 3)
114. Help designing primers; also help with viewing GenBank - (reply: 3)
115. Housekeeping gene validation - (reply: 7)
116. Primer optimization (with probes) - Urges... (reply: 2)
117. delta delta CT method - (reply: 3)
118. Dilute your cDNA ? - for real time PCR (reply: 2)
119. housekeeping gene for B cell lines - (reply: 1)
120. final extension real time PCR - (reply: 4)
92. Standard curve from plasmid - for cDNA quantification (reply: 6)
93. High baseline/noise probes - (reply: 2)
94. problems cleaning up small DNA products - (reply: 2)
95. interpretation of results of relative qRTPCR - please help (reply: 5)
96. New to qPCR - (reply: 2)
97. RNA from paraffin - (reply: 1)
98. Abgene PCR master mix?-MgCl concentration - (reply: 1)
99. Sybr Green real time pcr limit of detection - (reply: 1)
100. real-time PCR problem - (reply: 1)
101. Can i use old standard curve to determine gene concentration? - (reply: 1)
102. Inconsistent Real Time results- RT efficiency, high Ct values - (reply: 3)
103. Creating standard curve - (reply: 1)
104. Real-time PCR for mRNA stability - (reply: 2)
105. Cre genotyping - smeared results (reply: 1)
106. Standard Curve method RT PCR- - (reply: 2)
107. Troubleshooting in quantifying Genomic/WGA DNA - (reply: 5)
108. Large smear in PCR - (reply: 2)
109. kras mutation. best method for detection - (reply: 4)
110. developing real time PCR - (reply: 5)
111. First time Real TIme - (reply: 2)
112. qPCR - running taqman for endogenous control and SYBR for the gene - (reply: 2)
113. geNorm and normalising factor - (reply: 3)
114. Help designing primers; also help with viewing GenBank - (reply: 3)
115. Housekeeping gene validation - (reply: 7)
116. Primer optimization (with probes) - Urges... (reply: 2)
117. delta delta CT method - (reply: 3)
118. Dilute your cDNA ? - for real time PCR (reply: 2)
119. housekeeping gene for B cell lines - (reply: 1)
120. final extension real time PCR - (reply: 4)