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511. help PCR of GC rich templates - (reply: 3)
512. I am so disappointed that I failed in PCR again and again - (reply: 4)
513. My PCR product is 100bp (approx). What result is more reliable-gel or hybridisat - (reply: 5)
514. really stupid question... - (reply: 7)
515. determine cyp3a16 primer - mouse liver progenitor cell (reply: 5)
516. PCR contamination - (reply: 2)
517. amplicon which species does it belong to? - (reply: 6)
518. Melting curve analysis: bioinformatic question (DAN) - (reply: 4)
519. RT control product appears only 5 cycles away from unknown samples - (reply: 2)
520. Help! Opticon real-time machines thinks I only want to do 1 cycle! - (reply: 3)
521. 18S as hk, how can i optimise it? - (reply: 1)
522. No CT only dissociation curve, is it of any help? - (reply: 1)
523. "Undetermined" Ct, but product IS formed?!?! - (reply: 8)
524. Is there any easier way to make deletion and site-mutation at same time? - (reply: 4)
525. Relative expression of genes between bacterial species - (reply: 1)
526. 3 bands after digestion - (reply: 13)
527. primer : shifting peak - (reply: 4)
528. rt-pcr data and western blot data not compatible - what could be the explanation (reply: 5)
529. Designing primers and hybridization probes - (reply: 1)
530. Junk/dimers on left of single product peak - (reply: 3)
531. synthesis of long cDNA - (reply: 2)
532. Real Time advantages - (reply: 1)
533. Bad slopes in TaqMan reactions - (reply: 3)
534. Calculating efficiency of raw data - (reply: 2)
535. How can I prepare standard DNA for QPCR - (reply: 3)
536. Reference genes on same plate as gene of interest? - (reply: 2)
537. RT-PCR primer design - (reply: 1)
538. Primers work for regulary PCR but not real-time PCR - why? (reply: 8)
539. Where is my spike? - (reply: 2)
540. Real Time Contamination/Background Issues - (reply: 1)
512. I am so disappointed that I failed in PCR again and again - (reply: 4)
513. My PCR product is 100bp (approx). What result is more reliable-gel or hybridisat - (reply: 5)
514. really stupid question... - (reply: 7)
515. determine cyp3a16 primer - mouse liver progenitor cell (reply: 5)
516. PCR contamination - (reply: 2)
517. amplicon which species does it belong to? - (reply: 6)
518. Melting curve analysis: bioinformatic question (DAN) - (reply: 4)
519. RT control product appears only 5 cycles away from unknown samples - (reply: 2)
520. Help! Opticon real-time machines thinks I only want to do 1 cycle! - (reply: 3)
521. 18S as hk, how can i optimise it? - (reply: 1)
522. No CT only dissociation curve, is it of any help? - (reply: 1)
523. "Undetermined" Ct, but product IS formed?!?! - (reply: 8)
524. Is there any easier way to make deletion and site-mutation at same time? - (reply: 4)
525. Relative expression of genes between bacterial species - (reply: 1)
526. 3 bands after digestion - (reply: 13)
527. primer : shifting peak - (reply: 4)
528. rt-pcr data and western blot data not compatible - what could be the explanation (reply: 5)
529. Designing primers and hybridization probes - (reply: 1)
530. Junk/dimers on left of single product peak - (reply: 3)
531. synthesis of long cDNA - (reply: 2)
532. Real Time advantages - (reply: 1)
533. Bad slopes in TaqMan reactions - (reply: 3)
534. Calculating efficiency of raw data - (reply: 2)
535. How can I prepare standard DNA for QPCR - (reply: 3)
536. Reference genes on same plate as gene of interest? - (reply: 2)
537. RT-PCR primer design - (reply: 1)
538. Primers work for regulary PCR but not real-time PCR - why? (reply: 8)
539. Where is my spike? - (reply: 2)
540. Real Time Contamination/Background Issues - (reply: 1)