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1. comparative Ct method and statistical significance test - (reply: 2)
2. Which DNA is good for PCR for amplify a certain sequence? cDNA or genomic DNA ? - (reply: 1)
3. Amplification efficiency - (reply: 3)
4. Compare the expression of two target genes - (reply: 1)
5. PCR Inhibitor - Where/What are you? (reply: 8)
6. Validation calculations - (reply: 1)
7. RE sites on the primer. - How can I check for restriction sites on a primer sequence? (reply: 2)
8. What are normalisation primers for the rt-PCR? - Im going to order the mirVana rt-PCR kit for miRNAs (reply: 1)
9. qPCR delta delta CT calculation - (reply: 1)
10. Using Ct values below 10 - (reply: 3)
11. Two peaks in dissociation curve - Two peaks in dissociation curve (reply: 1)
12. Baseline and threshold values for a reference and target gene can be different? - (reply: 2)
13. House keeping gene copy number - (reply: 3)
14. PCR efficiencies for analysis of relative real time data - (reply: 1)
15. Mixing RT-PCT master mixes and plates - Best way to do it? (reply: 1)
16. Gene Expression level of apoptotic-related genes by qReal Time RT PCR - (reply: 1)
17. cDNA dilution for efficency calculation - (reply: 1)
18. "Gene expression vs Transcription vs mRNA levels": Help with conceptua - (reply: 1)
19. Primer design from mRNA or DNA - Confused about what sequence to use for design of GSP and pPCR primers (reply: 2)
20. Big Big problem with my Q-PCR results - (reply: 4)
21. Plexor HY: inconsistent results? - (reply: 2)
22. -RT works better than +RT? - (reply: 9)
23. Why is antagonist inducing the gene? - (reply: 1)
24. DeltaDelta Ct Relative or Comparative Quantitation - methods of relative quantitation for qPCR determining gene expression (reply: 3)
25. normalizing between qPCR runs - (reply: 2)
26. statistics - gene expression quantification (reply: 1)
27. non-linearity - Ct - (reply: 1)
28. qPCR plate setup - too many samples for one plate, how to divide samples? (reply: 1)
29. qPCR troubles - (reply: 5)
30. CT value of reerence gene - what should be the GAPDH Ct value (reply: 1)
2. Which DNA is good for PCR for amplify a certain sequence? cDNA or genomic DNA ? - (reply: 1)
3. Amplification efficiency - (reply: 3)
4. Compare the expression of two target genes - (reply: 1)
5. PCR Inhibitor - Where/What are you? (reply: 8)
6. Validation calculations - (reply: 1)
7. RE sites on the primer. - How can I check for restriction sites on a primer sequence? (reply: 2)
8. What are normalisation primers for the rt-PCR? - Im going to order the mirVana rt-PCR kit for miRNAs (reply: 1)
9. qPCR delta delta CT calculation - (reply: 1)
10. Using Ct values below 10 - (reply: 3)
11. Two peaks in dissociation curve - Two peaks in dissociation curve (reply: 1)
12. Baseline and threshold values for a reference and target gene can be different? - (reply: 2)
13. House keeping gene copy number - (reply: 3)
14. PCR efficiencies for analysis of relative real time data - (reply: 1)
15. Mixing RT-PCT master mixes and plates - Best way to do it? (reply: 1)
16. Gene Expression level of apoptotic-related genes by qReal Time RT PCR - (reply: 1)
17. cDNA dilution for efficency calculation - (reply: 1)
18. "Gene expression vs Transcription vs mRNA levels": Help with conceptua - (reply: 1)
19. Primer design from mRNA or DNA - Confused about what sequence to use for design of GSP and pPCR primers (reply: 2)
20. Big Big problem with my Q-PCR results - (reply: 4)
21. Plexor HY: inconsistent results? - (reply: 2)
22. -RT works better than +RT? - (reply: 9)
23. Why is antagonist inducing the gene? - (reply: 1)
24. DeltaDelta Ct Relative or Comparative Quantitation - methods of relative quantitation for qPCR determining gene expression (reply: 3)
25. normalizing between qPCR runs - (reply: 2)
26. statistics - gene expression quantification (reply: 1)
27. non-linearity - Ct - (reply: 1)
28. qPCR plate setup - too many samples for one plate, how to divide samples? (reply: 1)
29. qPCR troubles - (reply: 5)
30. CT value of reerence gene - what should be the GAPDH Ct value (reply: 1)