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Transformation Related Discussions
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Transformation of non-competent E. coli? - Any shortcut for preapring competent cells? (reply: 2)
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small transformant colonies - (reply: 1)
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Chemical transformation in 96 well MTP - How to make competent cells in MTPs (reply: 2)
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Competent Cells - (reply: 1)
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Calculating transformation efficiency? - (reply: 1)
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can you transform directly into liquid culture? - (reply: 2)
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pGL-3 vector transformation - (reply: 5)
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How to deal with so many false positive clones - Transfer linear DNA into E.coli competent cells... (reply: 2)
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Should I chuck my competent cells? probably contaminated.. - (reply: 4)
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Transformation Dilemma - I hope if you can figure out (reply: 5)
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ligation and transformation problems - (wrong) oligos (reply: 4)
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transformation of Saccharomyces cerevisiae - (reply: 1)
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transformation after miniprep - (reply: 7)
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Agrobacterium transformation problems - Trouble preparing agrobacterium (reply: 1)
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Transformation failure - (reply: 1)
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How many transformants should we select for screening? - SDM, QuikChange Lightning SDM Kit (reply: 1)
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Please help me on this ELISA analysis - Cannot figure out to transform OD into concentration (reply: 5)
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How to transform with topo vector - (reply: 6)
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transformation of polyploid industrial Saccharomyces - (reply: 5)
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Yeast Transformation Problems - (reply: 1)
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Transformation gone wrong! Please help! - (reply: 8)
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transformation issues with ligation product - a double-digested (HindIII / HpaI) vector won't take up an insert (reply: 5)
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Adding ethanol during transformation? - (reply: 1)
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How to select homozygous transformants? - Transgenic Arabidopsis (reply: 4)
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How long do I incubate after transformation? ? - (reply: 7)
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Problem with transformation - (reply: 4)
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how many plasmids can a competent bacteria take - (reply: 9)
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Transformation didnt work - (reply: 2)
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pBLuescript transformation - (reply: 1)
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Transformants pictures in gel - (reply: 2)
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Heat shock transformation... how does it works? - (reply: 5)
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Competent cells for transformation - (reply: 1)
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Transformation troubleshoot - (reply: 7)
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yeast transformation problem - (reply: 1)
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Transformation, no colonies! - (reply: 12)
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no pellet after centrifugation for making competent cells - (reply: 4)
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help with making ultra competent cell (Inoue method) - (reply: 3)
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making INV110 competent cells - (reply: 1)
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growth time of bacteria after transform - (reply: 2)
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Transfect competent cell? - (reply: 1)
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Improving transformation efficiency - large plasmids (reply: 6)
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pcr,ligation and transformation with RE SalI and XhoI - (reply: 4)
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PCR and competent cells - (reply: 3)
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TOP10 competent cell with another vector - (reply: 1)
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Overshot pH of Competent cell buffer (RbCl) :( - (reply: 3)
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NovaBlue(DE3) versus BL21(DE3): transformation problems? - (reply: 2)
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picking a cell line to perform cell transformation assays - (reply: 2)
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Glass Beads to plate bacteria transformation - (reply: 2)
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how to test the efficiency of chemically competent cells - (reply: 2)
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cloning and transformation - (reply: 1)
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Problems with making efficient chemically competent cells - (reply: 2)
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PEG removing A overhang? - low transformation efficiency (reply: 2)
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competent Bascillus subtilis cells - (reply: 2)
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purification、ligation and transformation problems - (reply: 11)
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ligation failure-what to do? - tried everything but still not getting transformants (reply: 12)
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ligation transformation.......which chemically competent cells are the best? - (reply: 3)
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E.coli transformation - (reply: 1)
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how to tell the transformation result? - (reply: 4)
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nucleotide mutations introduced during transformation - (reply: 3)
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Plasmid transformation - (reply: 4)
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Yeast Competent Cells - (reply: 34)
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Uncomplete plasmid after transformation - (reply: 1)
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Freezing transformations? - (reply: 1)
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Competent Cell Preparation - (reply: 2)
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Competent Listeria Cells - (reply: 3)
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Transformation of pCMV-sport6 vector to DH5 alpha competent cells - (reply: 1)
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Ligation and Transformation Problem - (reply: 2)
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Ligation, plasmid rescue problem! - PLasmid transformation after ligation problem (reply: 7)
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Tips on improving ligation+transformation from gel extraction - Let's contribute! (reply: 1)
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competent cell contamination? - (reply: 3)
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Transformation protocol for Bacillus subtilis needed - (reply: 1)
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Storage of transformation reaction mix (Promega) - CLoning-transformation (reply: 2)
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strange transformation products in subclone - (reply: 4)
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How can I transform GFP into recA -/- E. coli? - (reply: 1)
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Number of DNA entering during transformation - How many molecules of DNA are taken up during transformation (reply: 2)
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Competent cells not growing - (reply: 5)
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yeast transformation failed after doing it many times - (reply: 5)
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about heatshock transformation - (reply: 1)
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competent cell didn't grow in LB - (reply: 15)
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problems with E.coli heat shock transformations - (reply: 1)
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Problems in CaCl2 method ofr competent cells - (reply: 1)
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Electro-competent cells. How do I make them "more" competent? - Need advice on increasing the efficiency of Top 10 E. coli (reply: 10)
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pICZa transformation problem - (reply: 1)
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CaCl2 Competent Cell - (reply: 3)
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culturing trouble making competent cells - (reply: 1)
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Does clumpiness matter in soft agar assay (in vitro transformation assay)? - (reply: 1)
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Why Ligation and transformation not working? - (reply: 3)
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preparation of electroporation competent cells from chemically competent cells - (reply: 3)
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Transformation problem with newly ligated plasmids - (reply: 1)
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short oligos ligation and transformation - (reply: 3)
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Recombinant plasmid lost afer transformation - (reply: 3)
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why should the conversion efficiency of competent cell be at least 10^8 cfu/_ - (reply: 2)
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Question making competent bacterial cells - (reply: 2)
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competent agrobacteria - (reply: 2)
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Could competent cell be stored in liquid nitrogen? - (reply: 7)
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not getting transformants - (reply: 12)
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choosing competent cells - (reply: 5)
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I added 2.5 times as much LB to TSS (Transformation and Storage Buffer) - What would happen? (reply: 1)
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transformation (electroporation) - (reply: 2)
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What is the cause of my small E. coli colonies from transformation? - (reply: 7)
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Verifying Restriction sites generated in PCR of insert for subcloning - No colonies in transformation!Ligation issues?Insert issue? (reply: 4)
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How to prepare my own competent cells? - as above (reply: 5)
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Is it possible to transform competent cells with linear DNA? - (reply: 5)
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ligation transformation kit - fermentas kit (reply: 4)
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after transformation - (reply: 1)
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Confusing transformation results. - (reply: 5)
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Help -> Failed Transformations - (reply: 6)
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Ligation and transformation problem - (reply: 4)
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transformation competent cells problem - fastbac vectors (reply: 2)
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transformation of competent cells - (reply: 2)
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Transformation - (reply: 5)
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High Throughput transformation - (reply: 5)
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Solution for a Genome Problem - Calculation of Genome Transformation Probability (reply: 1)
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E. coli transformation issues - old plasmids (reply: 7)
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Chemically competent cells? - (reply: 3)
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chemical competent cells - From a Biojoe's pm (reply: 5)
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problems in ligation/transformation - (reply: 12)
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competent cells transform 12 kb plasmid,urgent - (reply: 8)
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Making competent cells from current commercial stocks - (reply: 3)
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transformation of T. reesei - (reply: 4)
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High transformation background - (reply: 3)
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Transformation efficiency? - (reply: 1)
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transformation of some uncommon bacterial - B licheniformis (reply: 2)
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Purchase or make competent cells? - (reply: 8)
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Ligation/ transforamtion troubles - (reply: 2)
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Very huge vector for transformation - (reply: 4)
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Trick in competent cell - make me crazy (reply: 8)
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Freeze-Thaw competent cells - (reply: 4)
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How much plasmid for transformation? - (reply: 5)
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transformation:more colonies with control! - control plate twice as many colonies as ligation (reply: 8)
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transformation failure - transformation failure (reply: 3)
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Inhibition of transformation by ligase - is it true? (reply: 3)
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Trying to transform without succes - (reply: 3)
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Help! E.coli transformation - (reply: 8)
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How about 4 DNA fragments ligation and cloning - Cloning by 4 DNA fragments ligation and transformation (reply: 6)
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making competent SURE cells - (reply: 2)
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Digestion, Ligation or transformation problem? - (reply: 8)
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bacterial transformation - (reply: 4)
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what is the mechanism of transformation of a plasmid into a bacterial cell? - (reply: 5)
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Weird transformation products - (reply: 5)
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Making Competent Cells - DH5A vs JM109 or anyother (reply: 5)
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ccdB competent cells - (reply: 2)
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Ligation at 16°C,ON: where to keep it till transformation? - (reply: 4)
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double digested PCR product into competent cell - (reply: 2)
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suspicious yeast - troubles with transformation during two hybrid assay (reply: 4)
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Transformation problems - time of transformation very low (0.7 ms) ???? (reply: 7)
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How to solve the problem of toxic ligation? - Are there any other competent cells better than DH5a dealing with toxi (reply: 3)
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Help w/ CLONING...lots of transformants but no insert... - (reply: 8)
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2 plasmids in one competent cell - is this possible? - Cloning query (reply: 9)
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Strange result after ligation and transformation - (reply: 2)
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Different sizes of PCR products from different competent cells - (reply: 4)
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medium for competent cell - SOB vs SOC (reply: 6)
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Curiosity: competent cell and glycerol - (reply: 7)
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DNA linear transformation failure - (reply: 2)
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Get rid of cell debris after transformation? - (reply: 1)
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HS competent cells in -20 - are they still usable (reply: 2)
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Agrobacterium tumefaciens 33970 mediated transformation of medicinal plant - Agrobacterium tumefaciens 33970 (reply: 8)
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crown gall / Agrobacterium tumefaciens 33970 - genetic transformation of some medicinal plant (reply: 2)
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Multiple transformation - How many plasmids is it possible to transform at the same time ? (reply: 2)
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Making DH10bac competent cells - (reply: 2)
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transformation troubles - where to go next? - transformation/ligation (reply: 11)
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weird DNA bands in the Minipreps; competent cells contamination? - (reply: 3)
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Transformation Frequency - (reply: 1)
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Transformation efficiency of plasmid dna - (reply: 2)
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Transformation of a plasmid into E.coli BL21 - problem! - Unexpected bands (reply: 7)
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why competent cells take up only 1 type of plasmids? - (reply: 7)
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Protocol Transformation in M. smegmatis - (reply: 1)
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yeast transformation efficiency issues - (reply: 4)
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transformation problem - (reply: 3)
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Transformation result - (reply: 1)
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Making frozen cell pellets competent - (reply: 3)
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High transformation background in negative control - (reply: 8)
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Troubleshooting Ligation/Transformation - (reply: 8)
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Chemically competent vs. Electrocompetent - (reply: 4)
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mutation during transformation? - (reply: 3)
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Strange Maxi-Prep problem - low yields with QuickChange mutagenesis competent E. Coli (reply: 1)
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Competent Cells Preparation - (reply: 9)
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Problems with Transformation - (reply: 4)
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transformation of competent cells with recombinant plasmids - (reply: 2)
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Ligation or transformation problem - (reply: 12)
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Turbo Competent Cells - (reply: 3)
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why do supercoiled plasmid transform faster than linear plasmid - (reply: 3)
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Usage of salmon sperm DNA in yeast transformation? - (reply: 3)
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Transformation for Pichia pastoris into yeast? - (reply: 2)
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Please help! Can Dh5alpha Competent cells be used for electroporation? - (reply: 8)
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Transformation problem. Help me! - (reply: 6)
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Tip for transformation : give me your opinion please! - (reply: 8)
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Problem with transformation using electroporator from eppendorf - (reply: 7)
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Hanahan Method for preparation of Competent cell - a little problem (reply: 2)
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Tips for transformation - which volume of ligation product? purification before transformation? Quantity o (reply: 4)
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competent cells - CaCl2 (reply: 2)
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DH5alpha transformation: protocol - (reply: 5)
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Is competent cell still good in -80oC for 16 months? - got a different plasmid after plasmid prep (reply: 3)
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transformation into pichia pastoris - no transformants with pPICZAalpha (reply: 4)
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plasmid size change after transformation - (reply: 1)
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Transformation efficiency - Help! (reply: 1)
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Problem in picking colonies after transformation - (reply: 9)
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toxic gene ligation/transformation URGENT HELP! - (reply: 1)
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Making competent E.coli is getting me desperate - After succeeding once, all tries after keep failing (reply: 13)
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problem in ligation and transformation - (reply: 18)
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Prepping and transformation - (reply: 2)
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Transformation using linearized plasmid? - (reply: 6)
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Transformation of a 21kb vector - (reply: 11)
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competent cell - (reply: 2)
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Transformation problems - (reply: 2)
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no colony after transformation of BL21(DE3),why? - (reply: 2)
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Glycerol wash protocol for chemical competent cells - (reply: 1)
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competent cells - making compentent cells (reply: 2)
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chemically competent cells - which method do you use? (reply: 7)
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Transformation by CaCl2 method - (reply: 1)
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calculation of transformation efficiency - (reply: 2)
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Transformation help - (reply: 3)
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Transformation efficiency - How to calculate!? (reply: 6)
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How to transform a 2.1kb fragment - (reply: 4)
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How to elude DNA sample from a paper? - and then transform E.coli... (reply: 2)
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Ligation and transformation - (reply: 3)
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no growth on plate agar for competent cell - (reply: 6)
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Only a few colonies after transformation - (reply: 7)
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To make good plasmid for expression transformation - (reply: 1)
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protoplasts transformation - (reply: 2)
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transformation step: 5min or 30min? - (reply: 10)
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Arabidopsis cells suspension transformation - (reply: 3)
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How to tell if competent cells or SOC are contaminated? - (reply: 14)
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what competent cells should I use to clone big size DNA - (reply: 8)
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Incubation of transformants - (reply: 5)
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ligation, transformation problem - (reply: 8)
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transformation of a big plasmid in E. coli - something wrong is happening in my transformation (reply: 6)
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DH5 alpha competent cells instead of M15? - competent cells for pQE30 construct (reply: 2)
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Transformation and ligation problems - (reply: 3)
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Able C and Able K competent cels - Has anyone use these cells before (reply: 2)
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To inactivate or not to inactivate ligase before transformation? - (reply: 5)
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ligation transformation of puc18 and phage lambda using ecor1 and bamh1 - general double/single digest problem (reply: 3)
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Troubleshooting ligation/transformation - (reply: 1)
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Why I keep getting no colonies from transformation of PCR products - help! (reply: 5)
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Ligation/Transformation problem - (reply: 4)
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Generation/Transformation into DY380 cells - (reply: 4)
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Transformation - (reply: 4)
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ligation to transformation - (reply: 1)
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What is spheroplasting transformation? - (reply: 2)
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TOPO cloning reaction transformation after 2 days - (reply: 1)
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My competent cells are misguiding me - (reply: 3)
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mysterious band after transformation and miniprep... - (reply: 11)
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Transduction, Transfection, Transformation and Infection - (reply: 7)
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How to produce competent celss XL1 Blue w/o inserts - (reply: 2)
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transformation colony - how to get rid of background colonies? (reply: 2)
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pVIB onto E. coli - bacterial transformation (reply: 2)
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E. coli transformation - (reply: 3)
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M15 transformation problem? - (reply: 1)
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making more chemically competent cells - (reply: 6)
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making competent cell - protocol molecular biology (reply: 8)
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Extremely lunatic competent cell - transformation (reply: 11)
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ligation/transformation issue - (reply: 5)
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e. coli transformation efficiency... - (reply: 1)
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Cloning - Electrocompetent vs chemically competent (reply: 1)
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Transformation problem (HELP!) - (reply: 4)
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Have you ever add IPTG to TOP10 cell (Invitrogen) - competent cell, IPTG (reply: 1)
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trouble shooting ligation and transform - (reply: 2)
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How to injure cell wall of chlorella for transformation - (reply: 2)
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Plasmid changes after transformation - (reply: 2)
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Competent cell - How good does it need to be? (reply: 4)
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Transformation efficiency and [DNA] - inhibition of transformation by DNA (reply: 1)
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How to sterilize disposable electroporation cuvettes - For Pichia Pastoris transformation (reply: 6)
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Strange results after transformation - (reply: 4)
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How do I make more competent cells? - by growing up and freezing glycerol stock? (reply: 39)
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competent cells question - (reply: 3)
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immortalization vs. transformation - (reply: 1)
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help on transformation - BAC clones transformation (reply: 4)
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transformation problems - (reply: 4)
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Store ligation reaction for repeat transformation? - (reply: 3)
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Transformation of an unstable gene - (reply: 1)
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blunt-sticky ligation/transformation problem - (reply: 6)
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Competent cell preparation - XL1-blue (reply: 4)
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DH5alpha transformation - poor transformation efficiency (reply: 2)
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Small, slow growing colonies from transformation - (reply: 6)
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Bacillus transformation - (reply: 1)
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mysterious 1.6kb band after transformation - (reply: 1)
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TA cloning - no colonies after transformation reaction (reply: 4)
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Cloning, ligation, transformation with pET - Problem re-ligating with NotI (reply: 1)
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Transformation of Yeast DNA into E.COli - (reply: 1)
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Thawing of competent bacterial cells - (reply: 2)
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Preparation of competent cells of pichia - (reply: 1)
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Why transformation at lower temp? - How does lower temp improves transformation efficiency? (reply: 1)
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Transformation of yeast - (reply: 4)
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Cloning problem - Digestion, ligation and transformation (reply: 6)
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Transformation Efficiency - (reply: 5)
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ligation and transformation problems! Urgent! - (reply: 20)
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Small competent cells - (reply: 3)
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ligation and transformation - (reply: 11)
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Need help with results of transformation... - (reply: 5)
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Transform coli with two or more plasmids - Transformation (reply: 1)
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Transformation help! Urgent - (reply: 2)
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difficult to find the transformates containing insert DNA - ligation, transformation (reply: 10)
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How little competent cell do you use for transformation - (reply: 2)
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TA cloning kit problem - No single colonies after transformation!!! (reply: 10)
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genomic library - low transformation (reply: 1)
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pBABE Comp. Cells - Special cells for transformation (reply: 3)
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Transformation and PCR questions - (reply: 2)
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transformation efficiency - (reply: 4)
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why the clone ware so small? - plamid transformation (reply: 1)
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yeast transformation question... - (reply: 1)
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low transformation efficiencywith... - (reply: 1)
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Transformation technique's and [abx] affect on plasmid yield - plasmid production and isolation (reply: 1)
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Re: Low phosphate media forBacillus/Bacillus transformation protocol - (reply: 1)
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Low phosphate media forBacillus/Bacillus transformation protocol - (reply: 1)
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How to transform the cells witht wo different plasmids - (reply: 3)
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Difficulties in transformation - (reply: 10)
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Competent Bascillus Cells - (reply: 1)
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Re: How to transform thecells with two different plasmids with d... - (reply: 1)
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yeast transforamtion - (reply: 2)