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Real-time PCR Related Discussions
  1. qPCR problems with chIP samples - (reply: 2)
  2. qPCR delta delta CT calculation - (reply: 1)
  3. qRT-PCR for pre- and pri-miRNAs - (reply: 2)
  4. DeltaDelta Ct Relative or Comparative Quantitation - methods of relative quantitation for qPCR determining gene expression (reply: 3)
  5. normalizing between qPCR runs - (reply: 2)
  6. mirVana™ qRT-PCR miRNA Detection versus miScript SYBR Green PCR kit - advantages and disadvantages (reply: 1)
  7. qPCR plate setup - too many samples for one plate, how to divide samples? (reply: 1)
  8. qPCR troubles - (reply: 5)
  9. qPCR 96 well plate - (reply: 2)
  10. What grade of primers for real-time TaqMan PCR assays? - (reply: 1)
  11. Calculation issue for inconsistent results in triplicates in Taqman rPCR. - (reply: 10)
  12. qRT-PCR primer design - (reply: 1)
  13. QPCR data anlaysis - (reply: 4)
  14. Problems with second use of diluted cDNA in qPCR - (reply: 4)
  15. qRT-PCR - data analysis - methods that DO NOT use a calibrator ! (reply: 3)
  16. First strand cDNA synthesis for qRT-PCR - (reply: 1)
  17. qRT-PCR control - (reply: 1)
  18. Difference between semi-q and qPCR - (reply: 2)
  19. Multichannel Pipette for qPCR plates - Effective manual pipetting of qPCR plates (reply: 1)
  20. Stratagene MX4000 qPCR plates to give away - UK only & preferably pick up in Cambridge (reply: 5)
  21. Magnetic beads in a QPCR rxn - (reply: 1)
  22. qPCR internal control - (reply: 3)
  23. unsolved qPCR problem! - (reply: 1)
  24. how to select a calibrator for relative gene expression in real-time PCR - also about the inconsistant about the triplicate of real-time PCR (reply: 6)
  25. qRT-PCR: how to average multiple runs? - (reply: 1)
  26. QPCR - (reply: 9)
  27. QPCR - (reply: 2)
  28. qPCR - (reply: 1)
  29. New to qPCR - (reply: 2)
  30. Low Ct value in ChIP qPCR - (reply: 4)
  31. real-time PCR problem - (reply: 1)
  32. Real-time PCR for mRNA stability - (reply: 2)
  33. qPCR - running taqman for endogenous control and SYBR for the gene - (reply: 2)
  34. qRT-PCR reproducibility of triplicates? - (reply: 6)
  35. miRNA real-time using Sybr green - (reply: 9)
  36. How to get perfect amplification peaks - Share my long QPCR troubleshooting experience (reply: 1)
  37. TaqMan probes specificity - I need to amplify and quantify specifically homologous genes (reply: 4)
  38. qPCR standard curve problem - (reply: 1)
  39. Reverse transcriptation for qPCR with some specific primers. Should I use primer - (reply: 2)
  40. how to optimize primer and cDNA concentration for real-time - (reply: 2)
  41. Problem with qPCR of ChIP input - (reply: 12)
  42. Using real-time to differentiate between SNPs - (reply: 5)
  43. Bad primer efficiency during multiplex qPCR - (reply: 1)
  44. Optimising Taqman probe - PCR is successful using SYBR but not with hydrolysis probe (reply: 3)
  45. What Ct value is accepted as expression? - qRT-PCR Ct values (reply: 3)
  46. qRT-PCR first timer, controls? - (reply: 9)
  47. PAGE with Realtime PCR product (Sybr Green) - Post-staining with ethidium bromide? (reply: 4)
  48. ChIP and qPCR - ChIP and qPCR (reply: 4)
  49. Ki67 IHC vs Real-time PCR - studying proliferation (reply: 1)
  50. Fluorescence level in real-time - (reply: 2)
  51. Taqman experiment wrongly use SYBGReen programme - (reply: 1)
  52. What are different ways of genotyping a transgenic mouse? - what is best way to do genotyping--PCR, qRT-PCR or RT-PCR (reply: 3)
  53. miRNA array data and QPCR data correlation - (reply: 6)
  54. need help on microRNA real-time PCR - (reply: 6)
  55. Taqman target gene and sybr green endogenous control? - (reply: 1)
  56. TaqMan single v duplexing - (reply: 5)
  57. Realtime PCR-negative control and dissociation curve! - (reply: 2)
  58. standard curve method for relative qPCR quantitation - (reply: 2)
  59. RNA extraction from laser capture microdissections - how to see low expressed genes from trought RT/qPCR on laser capture m (reply: 2)
  60. Primer concentration is quantitative Real-time PCR - (reply: 1)
  61. choosing real-time PCR machine - (reply: 13)
  62. iQ5 dynamic well factors for TaqMan probes - (reply: 5)
  63. Statistics help with real-time relative quantification (RQ) values - (reply: 2)
  64. Real-Time PCR and Housekeeping gene - (reply: 9)
  65. ChIP and qRT-PCR - (reply: 1)
  66. Controls for real-time PCR... again - (reply: 7)
  67. TaqMan - 18S curve not sigmoidal, replicates are different - (reply: 4)
  68. Taqman PCR master mix - (reply: 4)
  69. ChIP qPCR data analysis? - (reply: 9)
  70. help!real-time PCR to see gene's expression after treatment with cytokin - (reply: 4)
  71. absolute qPCR problem - (reply: 1)
  72. taqman probe design - (reply: 1)
  73. Simple QPCR question? - (reply: 1)
  74. Detection of LNA miRNA knock-down by qPCR? - (reply: 5)
  75. Taqman qpcr sensitivity - (reply: 1)
  76. Taqman Vs SYBR Green - new to qRT-PCR and trying to learn.... (reply: 1)
  77. RT-PCR Vs qRT-PCR help please! - (reply: 4)
  78. SYBR green concentration for qPCR - (reply: 2)
  79. PCR efficiency for qRT-PCR analysis - PCR efficiency for qRT-PCR analysis (reply: 4)
  80. qrt-pcr on bacterial transcripts - (reply: 1)
  81. Can I run q RT real-time PCR based on cDNA amount? - (reply: 1)
  82. qPCR on low to non-existant template - (reply: 4)
  83. elp with qPCR standard curve - (reply: 5)
  84. HELP with qPCR results - (reply: 3)
  85. QPCR primer Pairs - (reply: 1)
  86. realtime from different starting tissue masses - (reply: 1)
  87. qPCR Exp. Design - Reruning Exp. Control Samples - (reply: 3)
  88. Statistics: real-time RT-PCR - (reply: 4)
  89. QPCR versus regular PCR for Chip - (reply: 2)
  90. Could you run Taqman PCR on gel to see the band? - (reply: 1)
  91. what is meaning of true enrichment for chip qPCR? - (reply: 5)
  92. MULTIPLEX REAL-TIME PCR WITH SYBR-GREEN - (reply: 1)
  93. Help for Real-time PCR equipment choice - (reply: 4)
  94. TaqMan dual-labelled probe low fluorescence problems - (reply: 2)
  95. Scramble siRNA shows increase in gene expression by Taqman RTPCR - (reply: 1)
  96. urgent help-how to get amplification from very low target copy number and how to - "Taqman probe" qPCR and "rotor gene 6000" machine (reply: 4)
  97. qRT-PCR help! - (reply: 2)
  98. qRT-PCR and Northern blot - Is miRNA Northern blot necessary? (reply: 2)
  99. gene-specific Primer for qRT-PCR - (reply: 2)
  100. No signal in Real-time but in normal PCR?! - (reply: 2)
  101. gDNA contamination in realtime PCR - (reply: 4)
  102. need help to evaluate primer sets for SYBR green qPCR - (reply: 2)
  103. Real-time PCR low efficiency - (reply: 1)
  104. qPCR databases - (reply: 1)
  105. qPCR and plasmid positive controls - Why use plasmid positive controls? Are they the best? If so, are there (reply: 1)
  106. QPcr for alternatively spliced products - (reply: 4)
  107. What happens if different templates dilution in Real-time PCR reaction - (reply: 1)
  108. qPCR primer efficiancy - (reply: 2)
  109. ChIP and qPCR calculation ? - (reply: 8)
  110. Touchdown conditions for qPCR - (reply: 1)
  111. Amplimer length for TaqMan - (reply: 1)
  112. QPCR Quantitation of copy number - (reply: 1)
  113. taqman is giving initially raised then fall off amplification curve why? - changed template concentraion n baseline too.....but ? (reply: 2)
  114. ELISA in a real-time PCR plate - possible? (reply: 3)
  115. Using sybr green and Taqman probes on the same plate? - (reply: 1)
  116. What different of TaqMan probe and Molecular Beacon probe ? - Why a reporter of TaqMan will be cleave, but not for a reporter of Mol (reply: 2)
  117. genomic DNA for qPCR quantification - target sequence? - (reply: 1)
  118. looking for a website for a newbie to learn qPCR - (reply: 3)
  119. qPCR for microRNAs - minimal primer length? (reply: 6)
  120. Real-Time MSP - (reply: 1)
  121. preference for region of primer design for qRT-PCR - (reply: 1)
  122. qPCR housekeepers - primers or probes? - (reply: 1)
  123. QPCR Oligo Database of Pathogens - (reply: 1)
  124. what is CDS primer used as a control for QPCR in CHIP? - (reply: 2)
  125. Free sample from Takara. - All kind of polymerase for PCR and real-time PCR. (reply: 7)
  126. Taqman or SYBR? - (reply: 4)
  127. Two Tm peak in real-time but only one product on a gel? - What's goning on????? (reply: 4)
  128. real-time PCR products on gel look good but melting curve is horribel - (reply: 2)
  129. Real-time PCR optimization - (reply: 8)
  130. Free FastStart Universal Real-Time PCR Master Mixes from Roche - (reply: 7)
  131. Degenerated qPCR data after keeping the cDNA in the fridge. - (reply: 2)
  132. Real-time PCR....Making sense of it all? - (reply: 3)
  133. Real-time PCR vs. Northern Blotting / mRNA stability assays... - Advice from those who have experience with mRNA stability assays, plea (reply: 2)
  134. QPCR with MSP - (reply: 3)
  135. Real-time PCR to analyze DNA methylation - (reply: 3)
  136. Homemade TaqMan PCR Mastermix? - (reply: 1)
  137. qPCR using random primers - (reply: 4)
  138. Verification of real-time amplification specificity - (reply: 1)
  139. Problems with RNA extraction or QPCR for AI - (reply: 2)
  140. miRNA QPCR advice - taqman (reply: 3)
  141. Taqman probe Tm ASAP! - (reply: 1)
  142. No-RT control in qRT-PCR - (reply: 5)
  143. Taqman miRNA Assay Controls - not the usal question - (reply: 5)
  144. Longest Amplicon Using Real-Time PCR? - (reply: 1)
  145. validation of micrarray data by realtime pcr - (reply: 1)
  146. cDNA for real-time PCR - (reply: 3)
  147. DNase digestion step necessary for real-time RT-PCR? - (reply: 2)
  148. PCR - qPCR inconsistent - To explain differences between normal PCR and qPCR (reply: 6)
  149. normalization for real-time PCR - (reply: 4)
  150. One-step versus two-step qRT-PCR - (reply: 5)
  151. Help with ChIP analysis using Taqman - (reply: 1)
  152. primer for real-time and reverse transcriptase PCR - (reply: 1)
  153. Statistical analysis of quantitative real-time PCR (qRTPCR) - Statistics on qRTPCR data (reply: 2)
  154. Sybergreen vs taqman? - Comparable? (reply: 1)
  155. qPCR vs RNA protection assay - (reply: 1)
  156. Plasmid for qPCR standard curve - (reply: 3)
  157. Can you use Picogreen in a QPCR instrument? - (reply: 1)
  158. Efficiency greater than 100% using taqman probes? - (reply: 4)
  159. Real-Time PCR Question - (reply: 1)
  160. Target Amplicon Length for real-time PCR - (reply: 4)
  161. Real-Time PCR/Melting Curve Question - (reply: 3)
  162. can Primers for RT-PCR be used Real-Time PCR directly? - (reply: 2)
  163. Real-Time PCR Amplicon Question - (reply: 2)
  164. need advice on qPCR results (see attachment) - melting curve - gel picture (reply: 5)
  165. Diluting your cDNA for qRT-PCR? - (reply: 5)
  166. SYBR VS TAqman- What is the difference other than PRimers? - TAqman works and SYBR doesnt (reply: 1)
  167. RT-PCR - not qPCR! urgent please - how to choose primers (reply: 3)
  168. highly recommended company or etc for TaqMan probe in MethyLight - (reply: 3)
  169. Help! Opticon real-time machines thinks I only want to do 1 cycle! - (reply: 3)
  170. RealTime PCR of microRNAs - (reply: 9)
  171. ChIP primer for promoter region of GAPDH - for taqman analysis (reply: 2)
  172. Bad slopes in TaqMan reactions - (reply: 3)
  173. What about the BIO-RAD's real-time machine? - (reply: 2)
  174. How can I prepare standard DNA for QPCR - (reply: 3)
  175. Primers work for regulary PCR but not real-time PCR - why? (reply: 8)
  176. qPCR Analysis - Standard Error of Individual dCT vs Group average expression (reply: 4)
  177. Standard Curve Issues with ABI 7500 qPCR - (reply: 4)
  178. Pre-Preparation of qPCR plates - Pros and Cons (reply: 4)
  179. ChIP qPCR analysis - (reply: 2)
  180. Re-amplify PCR product by realtime PCR? - (reply: 13)
  181. housekeeping gene in real-time PCR - (reply: 2)
  182. Trouble with real-time PCR standard curve - (reply: 4)
  183. 2 melting peaks in real-time PCR - (reply: 1)
  184. qPCR with Corbett Rotor Gene 6000 - (reply: 3)
  185. Relative Quantification in real-time RT-PCR - (reply: 5)
  186. mirVana qRT-PCR with the Roche 1.5 LightCycler - (reply: 1)
  187. Inconsistent results from replicates in Taqman real-time PCR - (reply: 26)
  188. NTC's in RT or just qPCR? - (reply: 3)
  189. Can't repeat a perfect miRNA real-time standard curve - (reply: 2)
  190. qPCR Efficiencies over 100% - Looking for references (reply: 1)
  191. real-time PCR for quantitation of MSP - (reply: 3)
  192. qPCR, no RT controls always positive (as cDNA samples) - (reply: 4)
  193. Small amounts of RNA for qRT-PCR - (reply: 7)
  194. TAQMAN real time - what is this??? (reply: 4)
  195. qPCR Standard control - plasmid quantify (reply: 3)
  196. Plate setup- have I covered everything? - help- qRT-PCR newbie in state of confusion! (reply: 2)
  197. how long should qPCR product be? - 150~250bp? (reply: 2)
  198. Series dilution of RNA or cDNA for qPCR standard curve? - (reply: 1)
  199. Strange thing about RNA concentration and real-time melting curve - (reply: 4)
  200. random primers for qPCR - (reply: 3)
  201. QPCR, where to start? - optimizing primer and template (reply: 5)
  202. qPCR standards - (reply: 1)
  203. Taqman QPCR help - amplification of FAM right from the 0 cycle (reply: 5)
  204. TaqMan probes vs Molecular Beacon probes - (reply: 1)
  205. QPCR Copy number - (reply: 2)
  206. RT-qPCR of bacteria mRNA from sludge - RT-PCR, Real-time PCR, mRNA, sludge (reply: 3)
  207. Taqman versus SYBR Green - (reply: 2)
  208. qPCR detection for single nucleotide substitution - (reply: 5)
  209. Question about Realtime PCR primer concentration.... - (reply: 5)
  210. taqman assays and linreg - (reply: 1)
  211. Best product size for real-time RT-PCR - (reply: 4)
  212. Which housekeeping genes for qRT-PCR control? - (reply: 17)
  213. Replication of qRT-PCR experiments - (reply: 5)
  214. generation of gDNA Std curves in qPCR - ABI 7300 qPCR (reply: 1)
  215. Minimum amount of RNA for real-time PCR - (reply: 4)
  216. is cDNA concentration 200 ng/ul too high for qRT-PCR - (reply: 17)
  217. genomic dna in real-time pcr - (reply: 3)
  218. What's the function of UNG in qPCR? - (reply: 6)
  219. What should I do for more samples need to be tested by real-time PCR? - (reply: 1)
  220. ChIP quantification (qPCR) - (reply: 2)
  221. Not enough cDNA for qRT-PCR - how to increase cDNA yield or save cDNA? - (reply: 12)
  222. Self-made qRT-PCR enzyme-/mastermix? - (reply: 8)
  223. TaqMan probe - (reply: 4)
  224. Home-made Sybr green mix for real-time PCR - (reply: 1)
  225. Should I dilute cDNA for real-time PCR? - (reply: 3)
  226. Real-time PCR problems - RT_PCR (reply: 7)
  227. strange contamination in real-time PCR in negative control? - (reply: 1)
  228. Homemade RealTime Syb-GreenI-Mix - (reply: 23)
  229. How to calculate absolute fold change in expression by realtime PCR - Realtime PCR (reply: 2)
  230. Problem with QPCR - control gene does not amplify - (reply: 2)
  231. qPCR for HKG and traditional PCR for GOI - (reply: 1)
  232. Smeary bands in real-time RT-PCR - (reply: 7)
  233. Touch down real-time PCR? - (reply: 1)
  234. real-time slopes - (reply: 4)
  235. RNAi in plant and detect with real time PCR - Detect gene expression decrease with qRT-PCR (reply: 2)
  236. 1st time real-time PCR questions - (reply: 1)
  237. real-time pcr optimization problem - (reply: 4)
  238. Optimal size for qRt-PCR - (reply: 3)
  239. Taqman for low levels of a gene (LacZ gene)in tissue - (reply: 1)
  240. Primer Dimer problems in real-time PCR - (reply: 14)
  241. Has anyone used 8E5 cells as standard for HIV qPCR? - HIV standards (reply: 1)
  242. some qPCR questions (naive ones included)... - first pitfals etc... (reply: 8)
  243. Is RealTime PCR data enough for Telomerase activity? - (reply: 4)
  244. designing QRT-PCR Primers - (reply: 11)
  245. Optimizing primers for SYBR green qPCR? - Optimizing primers for SYBR green qPCR? (reply: 7)
  246. Another QPCR problem. Please help - (reply: 5)
  247. Trouble with standard curves for real-time PCR - (reply: 6)
  248. Real-time PCR machines for DNA quantitation - (reply: 5)
  249. qPCR after ChIP - lack of reproducibility - (reply: 9)
  250. TaqMan probes, pcr mix - (reply: 1)
  251. Use of ROX in qPCR - (reply: 8)
  252. strange QPCR amplificaton plot - (reply: 3)
  253. total RNA from BSE infected WBC for microarray and qPCR - (reply: 2)
  254. Mg curve for real-time PCR - Strange result (reply: 5)
  255. Northern vs qRT-PCR for expression quantitation - (reply: 6)
  256. Real-time PCR probe degradation - when do they degrades? (reply: 4)
  257. realtime pcr validating the results of microarray? - (reply: 7)
  258. My SYBRgreen qPCR stopped working - (reply: 3)
  259. AmpliTaq Gold instead of TaqMan Universal PCR Mix in Real-time? - (reply: 3)
  260. real-time thresholds - purpose? - (reply: 1)
  261. Real-time PCR - contamination or not? - (reply: 6)
  262. real-time PCR - Multiple melting curve peaks (reply: 18)
  263. MGB Taqman vs. Molecular Beacons - which is better for SNP discrimination? (reply: 1)
  264. Real-time PCR questions (Mx3000P) - Need some help with interpretation (reply: 7)
  265. QPCR books - (reply: 1)
  266. qRT-PCR data analysis - (reply: 1)
  267. qPCR and siRNA - (reply: 2)
  268. mastermix for Real-time PCR - (reply: 2)
  269. Real-Time qRT-PCR standard curves... efficiency is too high! - (reply: 19)
  270. qPCR standard curves - how can I keep the quality good? (reply: 6)
  271. Construct DNA plasmid for QPCR - (reply: 4)
  272. real-time PCR - (reply: 5)
  273. Realtime PCR standard curves - (reply: 8)
  274. Real-time PCR amplification curve up and down - (reply: 3)
  275. Which RT primer is better for qPCR - (reply: 2)
  276. Do I have amplication of RNA by qPCR? - (reply: 7)
  277. melt curves for SYBR Green real-time PCR driving me nuts!! - melt curves real-time PCR (reply: 6)
  278. real-time RT-PCR one cell - (reply: 2)
  279. taqman probe design for MSP - (reply: 3)
  280. Maximum copy number in Taqman - (reply: 1)
  281. real-time pcr plasmid standards - (reply: 4)
  282. QRT-PCR - (reply: 4)
  283. contamination in real-time PCR - (reply: 32)
  284. Shopping for real-time PCR thermal cyclers - iCycleriQ real-time system? (reply: 1)
  285. contamination in real-time pcr negative control - (reply: 12)
  286. Real-time PCR problems - (reply: 3)
  287. TaqMan Stopped Working - TaqMan Stopped Working (reply: 2)
  288. real-time PCR/ABI Prism 7700 - Std curve programming (reply: 1)
  289. Real-time PCR and high Ct value - (reply: 1)
  290. realtime RT-PCR/internal control - (reply: 3)
  291. Realtime RT-PCR problem - no product - (reply: 6)
  292. product size for real-time PCR primer - (reply: 3)
  293. Efficiency Real-Time PCR - Real-time PCR (reply: 3)
  294. Real-time quantification in Chromatin IP - (reply: 2)
  295. qPCR on genomic DNA - (reply: 2)
  296. Taqman SNP genotyping - Reduced reaction volume (reply: 1)
  297. Real-Time PCR - No plateau! (reply: 1)
  298. Transcription Rate - Measure Hn RNA using Taqman?? (reply: 1)
  299. qPCR Primer Design and Genomic DNA Amplification - (reply: 1)
  300. does anyone know real-time methyaltion pcr? - (reply: 1)
  301. taqman high CT's - (reply: 5)
  302. real-time PCR - How to design primers (reply: 2)
  303. About Q-PCR(real-time PCR) - COMPARE (reply: 1)
  304. QRT-PCR result analysis - relative transcript level? (reply: 3)
  305. Familiar with MX3000P by Stratagene?? - qPCR (reply: 1)
  306. Probes for Real-Time PCR - (reply: 3)
  307. taqman assay - contents (reply: 1)