taqman assays and linreg - (Apr/04/2006 )

Dear all,

Is there anyone out there who is doing Taqman assays and successfully using LinReg analysis tool for analysis?

I do one-step Taqman qRT-PCR assays on the Opticon-2. But, when I export raw data into LinReg tool all data points are negative and an error message appears "Invalid floating point operation". Has anyone experienced this? Any help is greatly appreciated.

Cheers,

qRT-PCR

I once tried LinReg and was faced with a similar problem. The decimal fractions in my LightCycler raw data was seperated with a decimal point instead of a comma (what my spread sheat uses). And the other time this problem appeared was with negative controls, that showed no amplification. These must be excluded from your data.

westenmax