Taqman PCR master mix - (Feb/21/2008 )

pls some one help me..wht different between taqman pcr master mx with AmpErase UNG and no AmpErase UNg..before this i used master mix without UNG but is it ok if i want to use master mix with UNG for the same sample..
tq

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i guess they are from ABI? should not make much difference, the AmpERASE UNG enzyme just prevents carry-over contamination from previous PCRs, at least as long as you don't have such contaminations.

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The AmpErase or dUTP-UNG system can only reduce the risk of carry-over contamination. It does not remove the contamination risk completely. In order to avaoid contamination, GLP is the only way.

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what is GLP?

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Good Laboratory Practice.

In this case all that will prevent cross contamination like physical separation of pre- and post-PCR activities and equipment, use of filter tips, etc.

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