Protocol Online logo
Top : Forum Archives: : Real-Time PCR

How to get perfect amplification peaks - Share my long QPCR troubleshooting experience (Jun/19/2008 )

Hi, I just completed my QPCR troubleshooting and finally got nice amplifications. I prepared some images at all the steps of my troubleshooting and uploaded here, just to share with you guys.

The bottom line: Use RNase-free tubes!

Good luck to everyone!

-wanglaoda-

QUOTE (wanglaoda @ Jun 19 2008, 10:16 PM)
Hi, I just completed my QPCR troubleshooting and finally got nice amplifications. I prepared some images at all the steps of my troubleshooting and uploaded here, just to share with you guys.

The bottom line: Use RNase-free tubes!

Good luck to everyone!


Thank you for sharing this with us!!!!

-Pallas-