SYBR green concentration for qPCR - (Jan/15/2008 )
Hi, does anyone know the concentration of SYBR green that's used for qPCR. SYBR green kits are already optimized so most people don't have to bother with this, but I have to try I "make my own" so any advice will be more than appreciated.
Thanks!!!
-almost a doctor-
1x SYBR Green I in real-time PCR = 1.96 uM.
Since SG I is sold as 10,000x, a preliminary dilution should be prepared in DMSO (for greatear stability) before preparing your 10x buffer with 10x SYBR Green I. Note that most SYBR master mixes contain extra magnesium.
-tfitzwater-
QUOTE (tfitzwater @ Jan 15 2008, 04:14 PM)
1x SYBR Green I in real-time PCR = 1.96 uM.
Since SG I is sold as 10,000x, a preliminary dilution should be prepared in DMSO (for greatear stability) before preparing your 10x buffer with 10x SYBR Green I. Note that most SYBR master mixes contain extra magnesium.
Since SG I is sold as 10,000x, a preliminary dilution should be prepared in DMSO (for greatear stability) before preparing your 10x buffer with 10x SYBR Green I. Note that most SYBR master mixes contain extra magnesium.
Thank you, will try that asap
-almost a doctor-