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Molecular-Biology
2761.
mutation study - DNA or mRNA sequence
(reply: 1)
2762.
how to remove a restricted endonuclease site -
(reply: 3)
2763.
substractive hybridization -
(reply: 2)
2764.
sequencing problem after pcr -
(reply: 4)
2765.
Amplifying plasmid - non specific binding of primer -
(reply: 3)
2766.
DNA folding to form the loop -
(reply: 2)
2767.
primer design -
(reply: 3)
2768.
Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (old and useful thread)
(reply: 2)
2769.
DNA purification - long fragments wanted -
(reply: 1)
2770.
want gene knock out protocol -
(reply: 2)
2771.
Unidentified DNA bands -
(reply: 1)
2772.
Problems digesting PCR product - troublesome enzymes: NotI PvuI
(reply: 2)
2773.
Accidentally digested DNA overnight - still OK? -
(reply: 4)
2774.
Shelf life of ampicillin in LB media? -
(reply: 6)
2775.
Make construct so it is only thing that can PCR after transfection -
(reply: 2)
2776.
Negative flouroscence in real time PCR -
(reply: 2)
2777.
Remove RNA after DNA mini-prep -
(reply: 1)
2778.
Minimum number of PCR cycles to see a product? -
(reply: 6)
2779.
Ligation after SalI & XhoI Digestion -
(reply: 3)
2780.
Rac1 mutants -
(reply: 1)
2781.
RNA from Sheep and Bovine blood - Isolation of RNA from bovine and ovine whole blood
(reply: 3)
2782.
decontamination - decontamination for PCR
(reply: 6)
2783.
Troubleshoot ARMS PCR -
(reply: 2)
2784.
Transfer of gene from one vector to another vector -
(reply: 2)
2785.
Transfection of vectors -
(reply: 8)
2786.
negative control for PCR -
(reply: 1)
2787.
Proteinase K to improve RNA extraction yield -
(reply: 2)
2788.
cloudy phase during RNA extraction with Trizol -
(reply: 10)
2789.
EtBr solution -
(reply: 3)
2790.
cDNA versus cRNA for a microarray -
(reply: 1)
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