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1711. fermentas clonejet pcr cloning kit - want opinion (reply: 1)
1712. Protein TAG for affinity purification - (reply: 1)
1713. Blunt-Cohesive ligation - Should I change the conditions? (reply: 2)
1714. GFP fused proteins - i'm going to cry... (reply: 3)
1715. what's the meaning? - HEAP (reply: 2)
1716. Help... my PCR don't come any more - PCR amplification desapeard (reply: 3)
1717. Making gels in advance - (reply: 2)
1718. Phenol-chloroform problem - (reply: 1)
1719. Cloning <100 bp fragments into a plasmid - (reply: 7)
1720. Plasmid miniprep w/ Glass fiber filter plates ???? - Protocol help (reply: 2)
1721. DNAse digestion while RNA extraction with TRIzol - anyone tried that before? (reply: 1)
1722. Amount of DNase to use (Qiagen) - (reply: 7)
1723. COMPLETE RNA degradation - (reply: 18)
1724. HpaI blunt end cloning - (reply: 1)
1725. undesired products in multiplex PCR - (reply: 3)
1726. Generate Supercoil Plasmid - it just a thought... (reply: 5)
1727. help with protein localization study using GFP - (reply: 6)
1728. Does a viral gene have introns? - (reply: 4)
1729. Selecting Lethal Mutants? - (reply: 1)
1730. RNA extraction from stained cells - (reply: 2)
1731. agarose gel electrophoresis - fluorescence which will not migrate from well and does not disappear a (reply: 5)
1732. No band after digestion - (reply: 4)
1733. Problem with ligation - Insert into the "wrong" vector (reply: 11)
1734. finding promoter - (reply: 1)
1735. Problem in expression of protein cloned in pcDNA3 - (reply: 1)
1736. Cloning in pCR2.1 plasmid - (reply: 7)
1737. How long can DNA precipitate at -20? - (reply: 6)
1738. Nucleotides with different font size - (reply: 2)
1739. primer sequence problem - (reply: 2)
1740. Maxiprep using alkaline lysis, very high OD, but low intensity on gel - (reply: 2)
1712. Protein TAG for affinity purification - (reply: 1)
1713. Blunt-Cohesive ligation - Should I change the conditions? (reply: 2)
1714. GFP fused proteins - i'm going to cry... (reply: 3)
1715. what's the meaning? - HEAP (reply: 2)
1716. Help... my PCR don't come any more - PCR amplification desapeard (reply: 3)
1717. Making gels in advance - (reply: 2)
1718. Phenol-chloroform problem - (reply: 1)
1719. Cloning <100 bp fragments into a plasmid - (reply: 7)
1720. Plasmid miniprep w/ Glass fiber filter plates ???? - Protocol help (reply: 2)
1721. DNAse digestion while RNA extraction with TRIzol - anyone tried that before? (reply: 1)
1722. Amount of DNase to use (Qiagen) - (reply: 7)
1723. COMPLETE RNA degradation - (reply: 18)
1724. HpaI blunt end cloning - (reply: 1)
1725. undesired products in multiplex PCR - (reply: 3)
1726. Generate Supercoil Plasmid - it just a thought... (reply: 5)
1727. help with protein localization study using GFP - (reply: 6)
1728. Does a viral gene have introns? - (reply: 4)
1729. Selecting Lethal Mutants? - (reply: 1)
1730. RNA extraction from stained cells - (reply: 2)
1731. agarose gel electrophoresis - fluorescence which will not migrate from well and does not disappear a (reply: 5)
1732. No band after digestion - (reply: 4)
1733. Problem with ligation - Insert into the "wrong" vector (reply: 11)
1734. finding promoter - (reply: 1)
1735. Problem in expression of protein cloned in pcDNA3 - (reply: 1)
1736. Cloning in pCR2.1 plasmid - (reply: 7)
1737. How long can DNA precipitate at -20? - (reply: 6)
1738. Nucleotides with different font size - (reply: 2)
1739. primer sequence problem - (reply: 2)
1740. Maxiprep using alkaline lysis, very high OD, but low intensity on gel - (reply: 2)