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New Forum Archives (2009-)
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Molecular-Biology
1681.
Primer Design - aligning sequences for probe/primer design
(reply: 2)
1682.
Cloning problem -
(reply: 2)
1683.
Lentivirus expression, no target protein - pHAGE-CMV-MCS-IZsGreen insert with S100B
(reply: 2)
1684.
3fragment ligation- troubleshooting - Cloning
(reply: 2)
1685.
In situ hybridization enigma -
(reply: 1)
1686.
A cloning nightmare -
(reply: 8)
1687.
Microtiter plate directed DNA degradation - Why is DNA on one plate degraded while intact on the next?
(reply: 3)
1688.
RNA fragmentation -
(reply: 3)
1689.
RNA extraction - how many cells do I need at least??
(reply: 4)
1690.
RNA extraction protocol for small amounts of cells -
(reply: 2)
1691.
about estrogen receptor beta negtive cell lines - can anyone give me some suggestion about ER beta negtive cell lines?
(reply: 1)
1692.
DNA sample contaminated with lipids -
(reply: 8)
1693.
cloning problem -
(reply: 5)
1694.
colony PCR inconsistent -
(reply: 4)
1695.
Newbie to Sequencing Question -
(reply: 1)
1696.
Problem with gel extraction of DNA. -
(reply: 1)
1697.
How to clone DNA (already cloned in a vector) in to another vector - please help
(reply: 20)
1698.
plasmid more than recommended for transfection - will it be toxic?
(reply: 1)
1699.
genomic DNA isolation -
(reply: 1)
1700.
E. coli not growing in liquid cultures :( -
(reply: 4)
1701.
Tricine SDS-PAGE for plant mitochondria - Membrane proteomics
(reply: 1)
1702.
DNA product almost 1500 bp -
(reply: 1)
1703.
Slightly too high 260:280 and 260:230 ratios from gDNA extraction - Should I care?
(reply: 2)
1704.
Blunt end filling - Blunt end filling
(reply: 1)
1705.
GHOST in Electrophoresis -
(reply: 18)
1706.
No luck with blunt and sticky ligations, need hlep - Prepare for a thorough read
(reply: 10)
1707.
Transformation background problem - high background in negative control
(reply: 9)
1708.
Akrylamide Gels -
(reply: 2)
1709.
Does unautoclaved DEPC inhibit northern blotting? -
(reply: 2)
1710.
Miniprep and sequencing -
(reply: 4)
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