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2611. Gel shift with large DNA fragments (>1 kb) - Can I still do EMSA? (reply: 1)
2612. re-amplification in real-time PCR - (reply: 1)
2613. 3'RACE and the polyA tail - why only one band? (reply: 2)
2614. midi-prep - (reply: 5)
2615. Degenerative primers = multiple products? - (reply: 5)
2616. 96 Well minipreps? - (reply: 1)
2617. DNAse activity in room temperature - (reply: 3)
2618. Reverse transcribing organically contaminated RNA - (reply: 2)
2619. Phylogenetic interpretation - (reply: 1)
2620. Do you lose RNA by quick freezing? - (reply: 5)
2621. obatining full nucelotide sequences for genes - where can i find them (reply: 4)
2622. No RNA from cell pellet using Qiagen MiniKit!!??!! - (reply: 5)
2623. Converting RE sites - (reply: 2)
2624. Can DNA be solubilized in hydrochloric acid? - (reply: 2)
2625. house keeping gene in transgenic mice. - (reply: 1)
2626. RNA cleanup methods (WITHOUT USING KITS!!) - protocol for removing phenol contamination (reply: 5)
2627. Detect gene in stable cell line, - (reply: 4)
2628. in vivo expression technology - IVET (reply: 2)
2629. basic math question - Molarity (reply: 2)
2630. EMSA supershift - increased band intensity? - (reply: 1)
2631. Who can explain this? Weird cloning problem.... - (reply: 6)
2632. RNA extraction - Which is which? (reply: 6)
2633. DNase I digestion - how long the fragments are at the end??? (reply: 3)
2634. Transfection efficiency vs. amount of plasmid DNA - (reply: 1)
2635. pIRESneo for mammalian cells transfection - (reply: 4)
2636. H9C2 - cell line - Need clarification (reply: 1)
2637. DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
2638. no band in gel - gel electrophoresis (reply: 1)
2639. stratagene site directed mutagenesis - deletion of single base nucleotide (reply: 3)
2640. How to increase A260/280 and A260/230 ratio for microarray experiment? - (reply: 8)
2612. re-amplification in real-time PCR - (reply: 1)
2613. 3'RACE and the polyA tail - why only one band? (reply: 2)
2614. midi-prep - (reply: 5)
2615. Degenerative primers = multiple products? - (reply: 5)
2616. 96 Well minipreps? - (reply: 1)
2617. DNAse activity in room temperature - (reply: 3)
2618. Reverse transcribing organically contaminated RNA - (reply: 2)
2619. Phylogenetic interpretation - (reply: 1)
2620. Do you lose RNA by quick freezing? - (reply: 5)
2621. obatining full nucelotide sequences for genes - where can i find them (reply: 4)
2622. No RNA from cell pellet using Qiagen MiniKit!!??!! - (reply: 5)
2623. Converting RE sites - (reply: 2)
2624. Can DNA be solubilized in hydrochloric acid? - (reply: 2)
2625. house keeping gene in transgenic mice. - (reply: 1)
2626. RNA cleanup methods (WITHOUT USING KITS!!) - protocol for removing phenol contamination (reply: 5)
2627. Detect gene in stable cell line, - (reply: 4)
2628. in vivo expression technology - IVET (reply: 2)
2629. basic math question - Molarity (reply: 2)
2630. EMSA supershift - increased band intensity? - (reply: 1)
2631. Who can explain this? Weird cloning problem.... - (reply: 6)
2632. RNA extraction - Which is which? (reply: 6)
2633. DNase I digestion - how long the fragments are at the end??? (reply: 3)
2634. Transfection efficiency vs. amount of plasmid DNA - (reply: 1)
2635. pIRESneo for mammalian cells transfection - (reply: 4)
2636. H9C2 - cell line - Need clarification (reply: 1)
2637. DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
2638. no band in gel - gel electrophoresis (reply: 1)
2639. stratagene site directed mutagenesis - deletion of single base nucleotide (reply: 3)
2640. How to increase A260/280 and A260/230 ratio for microarray experiment? - (reply: 8)