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991. Transformation /Cloning Problem - (reply: 3)
992. Ligation Problem - (reply: 4)
993. a robust multi-gene cloning method - (reply: 2)
994. I've run out of ideas.... Restriction enzyme digestion - (reply: 6)
995. Colony lift hybridisation positive but clones are negative for insert DNA - (reply: 1)
996. Mortar and Pestle for RNA Extraction - (reply: 1)
997. Any free software for drawing genes? - (reply: 9)
998. TREx pcDNA6/TR - (reply: 2)
999. Plasmid, gene orientation, insert orientation. - (reply: 1)
1000. Need stable GFP expression in IPS and embryonic SCs - (reply: 1)
1001. good tutorials/protocols/books on gene cloning - (reply: 2)
1002. How to clean extracted faecal DNA? (removing PCR inhibitors) - (reply: 1)
1003. yeast growth in SC-ura medium - (reply: 1)
1004. Optimizing Trizol mRNA extraction - (reply: 3)
1005. Expression in trans - (reply: 1)
1006. DNA Storage - (reply: 10)
1007. RBC LYSIS BUFFER - a good substitute of sucrose? - (reply: 5)
1008. Luciferase assay - expected range of raw reading values - (reply: 1)
1009. compare between 2 or more genes - (reply: 1)
1010. sample preservation for RNA extraction - (reply: 1)
1011. Designing primers for the ORFs - (reply: 9)
1012. Bioinformatics Software - (reply: 2)
1013. Low yields with quiagen midi kit - (reply: 2)
1014. RNA extraction from blood stain - (reply: 1)
1015. Knocking out non-essential gene - (reply: 2)
1016. PCR with phophorylated primers - (reply: 1)
1017. Nanodrop absorbtion at 290 nm??? - (reply: 1)
1018. Plasmid sequencing without primers - (reply: 1)
1019. Either too low or too high a260/a230 - (reply: 5)
1020. RNA purity - (reply: 3)
992. Ligation Problem - (reply: 4)
993. a robust multi-gene cloning method - (reply: 2)
994. I've run out of ideas.... Restriction enzyme digestion - (reply: 6)
995. Colony lift hybridisation positive but clones are negative for insert DNA - (reply: 1)
996. Mortar and Pestle for RNA Extraction - (reply: 1)
997. Any free software for drawing genes? - (reply: 9)
998. TREx pcDNA6/TR - (reply: 2)
999. Plasmid, gene orientation, insert orientation. - (reply: 1)
1000. Need stable GFP expression in IPS and embryonic SCs - (reply: 1)
1001. good tutorials/protocols/books on gene cloning - (reply: 2)
1002. How to clean extracted faecal DNA? (removing PCR inhibitors) - (reply: 1)
1003. yeast growth in SC-ura medium - (reply: 1)
1004. Optimizing Trizol mRNA extraction - (reply: 3)
1005. Expression in trans - (reply: 1)
1006. DNA Storage - (reply: 10)
1007. RBC LYSIS BUFFER - a good substitute of sucrose? - (reply: 5)
1008. Luciferase assay - expected range of raw reading values - (reply: 1)
1009. compare between 2 or more genes - (reply: 1)
1010. sample preservation for RNA extraction - (reply: 1)
1011. Designing primers for the ORFs - (reply: 9)
1012. Bioinformatics Software - (reply: 2)
1013. Low yields with quiagen midi kit - (reply: 2)
1014. RNA extraction from blood stain - (reply: 1)
1015. Knocking out non-essential gene - (reply: 2)
1016. PCR with phophorylated primers - (reply: 1)
1017. Nanodrop absorbtion at 290 nm??? - (reply: 1)
1018. Plasmid sequencing without primers - (reply: 1)
1019. Either too low or too high a260/a230 - (reply: 5)
1020. RNA purity - (reply: 3)