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2671. Good RT-PCR amplication but NO amplification with semi-quantitative PCR!? Wh - (reply: 3)
2672. gel extraction protocol for small amount of starting material - (reply: 3)
2673. Taq introduces error after? - (reply: 4)
2674. nylon membranes bleaching - (reply: 2)
2675. DNA vs Protein Stains - Are there any that don't stain BOTH? (reply: 1)
2676. Plasmid isolation, help with dilution step? - (reply: 7)
2677. Extraction of large size genomic DNA - (reply: 3)
2678. full digestion of plasmid to get only dNTPs - (reply: 3)
2679. Incorporate biotinylated-dNTPs by a polymerase - biotin (reply: 1)
2680. multiple bands in my RNA sample? - (reply: 2)
2681. expession only in +ve controls - (reply: 4)
2682. splice variant encoding same protein - (reply: 1)
2683. EtBr contamination risk - safety question (reply: 4)
2684. WHich primer to use for sequencing - (reply: 5)
2685. types of gel stains - (reply: 7)
2686. minimum length for the gene to be amplified in PCR - (reply: 6)
2687. wrong gene - (reply: 13)
2688. pheno chloroform step - (reply: 6)
2689. dNTP Quantity - (reply: 3)
2690. gene design - restriction site addition (reply: 2)
2691. 5' RACE products appearance on 1.5% agarose gel - (reply: 8)
2692. DNA Question for TV Show - (reply: 4)
2693. Amplification dwindles using little template RT-PCR - (reply: 3)
2694. Phenol chloroform extraction of DNA - (reply: 3)
2695. Number of genes and number of proteins in humans - (reply: 1)
2696. Degenerate PCR Size Limit? - (reply: 3)
2697. RNA isolation form plants - (reply: 4)
2698. How to increase the concentration insert or vector or no cut plasmid - How to increase the concentration insert or vector or no cut plasmid (reply: 4)
2699. 5' end RNA biotin labeling - need protocol (reply: 2)
2700. Yeast complementation test - (reply: 1)
2672. gel extraction protocol for small amount of starting material - (reply: 3)
2673. Taq introduces error after? - (reply: 4)
2674. nylon membranes bleaching - (reply: 2)
2675. DNA vs Protein Stains - Are there any that don't stain BOTH? (reply: 1)
2676. Plasmid isolation, help with dilution step? - (reply: 7)
2677. Extraction of large size genomic DNA - (reply: 3)
2678. full digestion of plasmid to get only dNTPs - (reply: 3)
2679. Incorporate biotinylated-dNTPs by a polymerase - biotin (reply: 1)
2680. multiple bands in my RNA sample? - (reply: 2)
2681. expession only in +ve controls - (reply: 4)
2682. splice variant encoding same protein - (reply: 1)
2683. EtBr contamination risk - safety question (reply: 4)
2684. WHich primer to use for sequencing - (reply: 5)
2685. types of gel stains - (reply: 7)
2686. minimum length for the gene to be amplified in PCR - (reply: 6)
2687. wrong gene - (reply: 13)
2688. pheno chloroform step - (reply: 6)
2689. dNTP Quantity - (reply: 3)
2690. gene design - restriction site addition (reply: 2)
2691. 5' RACE products appearance on 1.5% agarose gel - (reply: 8)
2692. DNA Question for TV Show - (reply: 4)
2693. Amplification dwindles using little template RT-PCR - (reply: 3)
2694. Phenol chloroform extraction of DNA - (reply: 3)
2695. Number of genes and number of proteins in humans - (reply: 1)
2696. Degenerate PCR Size Limit? - (reply: 3)
2697. RNA isolation form plants - (reply: 4)
2698. How to increase the concentration insert or vector or no cut plasmid - How to increase the concentration insert or vector or no cut plasmid (reply: 4)
2699. 5' end RNA biotin labeling - need protocol (reply: 2)
2700. Yeast complementation test - (reply: 1)