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1981. Anybody did any AscI ligation? - (reply: 5)
1982. Annealing Temperature of biotinylated primers - (reply: 2)
1983. RT-PCR - New to RT-PCR info (reply: 2)
1984. Help: Possible ligation of multiple plasmids when only self-ligation is wanted - How to differentiate the two? (reply: 5)
1985. Genomic DNA - (reply: 2)
1986. No RIN obtained with Bioanalyzer? - (reply: 2)
1987. Sequence, question! - (reply: 8)
1988. PCR failed no product.. help - (reply: 10)
1989. number of molecules in PCR - (reply: 3)
1990. Cloning Problem (cells grow but no plasmid isolated) - (reply: 13)
1991. EMSA - how to make long probes ? (reply: 4)
1992. point mutation when transformed in BL21 - (reply: 2)
1993. Primer dilution - PCR primer dilution (reply: 2)
1994. DNA quantification: NanoDrop vs. Picogreen - measurement of ChIP Input DNA (reply: 9)
1995. PCR reagent autoclave - (reply: 2)
1996. weird site directed mutagenesis products - (reply: 3)
1997. extraction of viral rna from plant tissue - (reply: 1)
1998. ampicillin for LB agar - (reply: 2)
1999. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
2000. PCR not working - overhangs too long? (reply: 3)
2001. What melting temperature to use - (reply: 1)
2002. PFGE Current? - (reply: 1)
2003. Plasmid frameshift problem after clonning - (reply: 2)
2004. Nde1-BamH1 makes me sad - high background despite DIFFERENT methods (reply: 34)
2005. TA cloning necessary? - (reply: 2)
2006. Is DNA an acid because of phosphate group - (reply: 2)
2007. Best methods for storing cDNA - Water, TE buffer, Tris ....... (reply: 3)
2008. colony formation assay - how many cells to seed (reply: 2)
2009. Conversion of primer unit - ug/ul to uM (reply: 4)
2010. mutagenesis by PCR or just use a kit - (reply: 5)
1982. Annealing Temperature of biotinylated primers - (reply: 2)
1983. RT-PCR - New to RT-PCR info (reply: 2)
1984. Help: Possible ligation of multiple plasmids when only self-ligation is wanted - How to differentiate the two? (reply: 5)
1985. Genomic DNA - (reply: 2)
1986. No RIN obtained with Bioanalyzer? - (reply: 2)
1987. Sequence, question! - (reply: 8)
1988. PCR failed no product.. help - (reply: 10)
1989. number of molecules in PCR - (reply: 3)
1990. Cloning Problem (cells grow but no plasmid isolated) - (reply: 13)
1991. EMSA - how to make long probes ? (reply: 4)
1992. point mutation when transformed in BL21 - (reply: 2)
1993. Primer dilution - PCR primer dilution (reply: 2)
1994. DNA quantification: NanoDrop vs. Picogreen - measurement of ChIP Input DNA (reply: 9)
1995. PCR reagent autoclave - (reply: 2)
1996. weird site directed mutagenesis products - (reply: 3)
1997. extraction of viral rna from plant tissue - (reply: 1)
1998. ampicillin for LB agar - (reply: 2)
1999. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
2000. PCR not working - overhangs too long? (reply: 3)
2001. What melting temperature to use - (reply: 1)
2002. PFGE Current? - (reply: 1)
2003. Plasmid frameshift problem after clonning - (reply: 2)
2004. Nde1-BamH1 makes me sad - high background despite DIFFERENT methods (reply: 34)
2005. TA cloning necessary? - (reply: 2)
2006. Is DNA an acid because of phosphate group - (reply: 2)
2007. Best methods for storing cDNA - Water, TE buffer, Tris ....... (reply: 3)
2008. colony formation assay - how many cells to seed (reply: 2)
2009. Conversion of primer unit - ug/ul to uM (reply: 4)
2010. mutagenesis by PCR or just use a kit - (reply: 5)