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Molecular-Biology
661.
improper recombination in transgenic mice -
(reply: 1)
662.
contamination of human cell lines with yeast -
(reply: 3)
663.
Barcoding PCR products -
(reply: 18)
664.
Re-culturing empty plasmid -
(reply: 1)
665.
IMAC nickel solution -
(reply: 1)
666.
Large scale Viral RNA purification Kit -
(reply: 3)
667.
UTR sequences after IRES -
(reply: 1)
668.
Storing Bacterial Culture -
(reply: 1)
669.
MTT: for 5 days -
(reply: 1)
670.
T7 promoter ending; to add or not to add GGG -
(reply: 2)
671.
Cytoplasmic mRNA isolation protocol -
(reply: 1)
672.
BSA in PCR reaction -
(reply: 1)
673.
Adding T7 promoter sequence to vector -
(reply: 2)
674.
Can CMV promoter start transcription in prokaryote cells -
(reply: 1)
675.
Taq vs Phusion polymerases - specificity -
(reply: 8)
676.
RNA isolation of murine pancreatic islets - poor ratios -
(reply: 3)
677.
Tagging a gene with HA and FLAG using PCR -
(reply: 1)
678.
Atypical yeild of RNA -
(reply: 1)
679.
How to join two sequencing files taken form forward and reverse primer -
(reply: 3)
680.
DNA EtOH/Sodium Acetate precip over the weekend...still good? -
(reply: 2)
681.
What happens to DNA in the presence of a chaotropic salt? -
(reply: 3)
682.
Lipofectamin 2000 and Large Plasmid Transfection Optimization -
(reply: 3)
683.
Problems with RNA extraction from Sorghum anthers/pollen -
(reply: 1)
684.
pcr product digestion problem -
(reply: 4)
685.
How to find a promoter and TSS? -
(reply: 1)
686.
A substitute for Triton-X100 in DNA extraction -
(reply: 9)
687.
Does Proteinase K digest RNAse in lysis buffer? -
(reply: 2)
688.
Third round PCR -
(reply: 2)
689.
how to amplify a fungus which is in the host tissue -
(reply: 3)
690.
RNA pellet gone in the wrong concentration of ethanol -
(reply: 4)
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