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481. a smeared Gen-DNA template ---> smear and less yield on pcr ? - (reply: 1)
482. PCR amplification of large template - (reply: 1)
483. Bacteria grew on plates but not in liquid culture - (reply: 1)
484. Doubt: genomic dna library construction - (reply: 3)
485. DH5alpha transformation protocol - (reply: 1)
486. Strange Negative Contamination Issue. - (reply: 2)
487. smear in gel electrophoresis after PCR - (reply: 2)
488. How to quantify MS2 virus-like particles? - (reply: 1)
489. Drugs that cause DNA double strand breaks - (reply: 1)
490. Blue middle tint in white colonies for Blue/White screening - (reply: 2)
491. SBI Minicircle DNA Kit -- No colonies -- ligation / transformation problem - (reply: 8)
492. No DNA after gel extraction - (reply: 9)
493. Cloning synthesised genes - (reply: 1)
494. DNA isolation from nuclei - (reply: 5)
495. DNA in 1water:0,1TE at 37 degrees - (reply: 1)
496. Why do people typically use AP instead of HRP for Southerns? - (reply: 2)
497. Difference between streptavidin-bound nucleic acid probe and probe covalently at - (reply: 5)
498. Low Transformation Efficiency with a Specific Gene - (reply: 4)
499. Plasmid DNA contamination - (reply: 5)
500. Problems with plasmid DNA extraction - (reply: 3)
501. Newbie to smaI blunt end cloning! - (reply: 1)
502. What part of the promoter to clone - (reply: 2)
503. PCR RFLP is PCR product purification necessary? - (reply: 3)
504. Biotinylated RNA on Nylon Membrane - (reply: 2)
505. conditionally active plasmids - (reply: 1)
506. UV crosslinkers, changing UV bulbs? - (reply: 1)
507. percentage DNA fragmentation - (reply: 1)
508. Weird gel banding for apoptosis - (reply: 3)
509. Buying pipettes, your opinion please - (reply: 8)
510. Co-IP vs Pull down - (reply: 2)
482. PCR amplification of large template - (reply: 1)
483. Bacteria grew on plates but not in liquid culture - (reply: 1)
484. Doubt: genomic dna library construction - (reply: 3)
485. DH5alpha transformation protocol - (reply: 1)
486. Strange Negative Contamination Issue. - (reply: 2)
487. smear in gel electrophoresis after PCR - (reply: 2)
488. How to quantify MS2 virus-like particles? - (reply: 1)
489. Drugs that cause DNA double strand breaks - (reply: 1)
490. Blue middle tint in white colonies for Blue/White screening - (reply: 2)
491. SBI Minicircle DNA Kit -- No colonies -- ligation / transformation problem - (reply: 8)
492. No DNA after gel extraction - (reply: 9)
493. Cloning synthesised genes - (reply: 1)
494. DNA isolation from nuclei - (reply: 5)
495. DNA in 1water:0,1TE at 37 degrees - (reply: 1)
496. Why do people typically use AP instead of HRP for Southerns? - (reply: 2)
497. Difference between streptavidin-bound nucleic acid probe and probe covalently at - (reply: 5)
498. Low Transformation Efficiency with a Specific Gene - (reply: 4)
499. Plasmid DNA contamination - (reply: 5)
500. Problems with plasmid DNA extraction - (reply: 3)
501. Newbie to smaI blunt end cloning! - (reply: 1)
502. What part of the promoter to clone - (reply: 2)
503. PCR RFLP is PCR product purification necessary? - (reply: 3)
504. Biotinylated RNA on Nylon Membrane - (reply: 2)
505. conditionally active plasmids - (reply: 1)
506. UV crosslinkers, changing UV bulbs? - (reply: 1)
507. percentage DNA fragmentation - (reply: 1)
508. Weird gel banding for apoptosis - (reply: 3)
509. Buying pipettes, your opinion please - (reply: 8)
510. Co-IP vs Pull down - (reply: 2)