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601. site-directed mutagenesis - (reply: 20)
602. qPCR detection - (reply: 2)
603. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
604. TCA precipitation - (reply: 1)
605. SOC vs TB in transformations - (reply: 1)
606. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
607. Single RE site cloning - (reply: 3)
608. PCR product running on agarose gel - (reply: 32)
609. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
610. PCR amplification with high fidelity enzyme - (reply: 1)
611. Transformation problem - (reply: 5)
612. finding gene sequence to delete - (reply: 4)
613. Blue fluorescent color of agarose gel in UV - (reply: 7)
614. heterologous gene integration into e.coli genome - (reply: 1)
615. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
616. Can anyone vouch for a protocol to get good gDNA from fungi? - (reply: 2)
617. Successful Transformation->Getting Colongy--> Getting DNA after miniprep - (reply: 9)
618. Qiagen column eluted DNA gives higher concetration than the original sample - (reply: 10)
619. Why do only some nuclear lysis buffers create DNA 'goop' - (reply: 1)
620. Continuous growth of E. coli on AMP LB plate - (reply: 2)
621. Plasmid compatibly groups - (reply: 5)
622. too much DNA on agarose gel - (reply: 10)
623. What are the "mammalian vectors N1 and C1"? - (reply: 1)
624. Life before BLAST etc? Help needed... - (reply: 1)
625. How base pairs upstream of gene to include for the promoter? - (reply: 3)
626. Neomycin resistance sequence - (reply: 1)
627. Can I use Real-time PCR instead of Southern Blot to determine number of integran - (reply: 1)
628. polyacrylamide RNA gel - (reply: 4)
629. Making a standard curve from bacterial suspensions - (reply: 1)
630. Is my RNA degraded? - (reply: 7)
602. qPCR detection - (reply: 2)
603. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
604. TCA precipitation - (reply: 1)
605. SOC vs TB in transformations - (reply: 1)
606. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
607. Single RE site cloning - (reply: 3)
608. PCR product running on agarose gel - (reply: 32)
609. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
610. PCR amplification with high fidelity enzyme - (reply: 1)
611. Transformation problem - (reply: 5)
612. finding gene sequence to delete - (reply: 4)
613. Blue fluorescent color of agarose gel in UV - (reply: 7)
614. heterologous gene integration into e.coli genome - (reply: 1)
615. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
616. Can anyone vouch for a protocol to get good gDNA from fungi? - (reply: 2)
617. Successful Transformation->Getting Colongy--> Getting DNA after miniprep - (reply: 9)
618. Qiagen column eluted DNA gives higher concetration than the original sample - (reply: 10)
619. Why do only some nuclear lysis buffers create DNA 'goop' - (reply: 1)
620. Continuous growth of E. coli on AMP LB plate - (reply: 2)
621. Plasmid compatibly groups - (reply: 5)
622. too much DNA on agarose gel - (reply: 10)
623. What are the "mammalian vectors N1 and C1"? - (reply: 1)
624. Life before BLAST etc? Help needed... - (reply: 1)
625. How base pairs upstream of gene to include for the promoter? - (reply: 3)
626. Neomycin resistance sequence - (reply: 1)
627. Can I use Real-time PCR instead of Southern Blot to determine number of integran - (reply: 1)
628. polyacrylamide RNA gel - (reply: 4)
629. Making a standard curve from bacterial suspensions - (reply: 1)
630. Is my RNA degraded? - (reply: 7)