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2401. Temp Gradient PCR - (reply: 2)
2402. BamHI and HindIII double digest - driving me mad... (reply: 1)
2403. NEB Restriction Enzymes and Buffer - Restriction (reply: 13)
2404. rna isolation - (reply: 1)
2405. DNA extraction with expired kit? - (reply: 1)
2406. Transcription of double blunt end Clone - (reply: 2)
2407. PCR with genomic dna - (reply: 9)
2408. mutagenesis - (reply: 1)
2409. Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
2410. PCR with long primers - (reply: 3)
2411. Need Help: Bacterial gene deletion- sacB sucrose resistance - (reply: 31)
2412. Gateway cloning - (reply: 8)
2413. RNA Isolation - RNA isolation from human cell lines (reply: 2)
2414. Which red fluorescence is better? - mCherry of dsRed (reply: 2)
2415. Finding corresponding amino acid in DNA sequence - (reply: 1)
2416. Buffer for 5 Kb DNA fragments - (reply: 2)
2417. Pooling doesn't work - make up rna amount of 10ug (reply: 3)
2418. Western Blot for Bacteria - Loading Control (reply: 1)
2419. uggly, non-focus and diffuse bands in my Western.. - Can you see my image of my Western to LC3? (reply: 1)
2420. Southern blotting - copy number determination (reply: 1)
2421. RNase: RNA ratio - making dsRNA (reply: 1)
2422. I hate my Klenow! - (reply: 9)
2423. Tris Base Buffer is the same that Trizma??No I think.. - Buffers:Differences between Tris Base and Trizma.. (reply: 3)
2424. Sequencing doubts - (reply: 3)
2425. Help with Northern Blotting - (reply: 4)
2426. ELISA - how can i get Mab for NS1? (reply: 1)
2427. 0.5% crystal violet - how to make (reply: 2)
2428. Inversed PCR issues - DNA isolation, restriction, ligation and PCR with no success (reply: 1)
2429. cDNA 260/280 ratio - (reply: 3)
2430. Amino acid substitution a problem? - Phenylalanine to leucine (reply: 3)
2402. BamHI and HindIII double digest - driving me mad... (reply: 1)
2403. NEB Restriction Enzymes and Buffer - Restriction (reply: 13)
2404. rna isolation - (reply: 1)
2405. DNA extraction with expired kit? - (reply: 1)
2406. Transcription of double blunt end Clone - (reply: 2)
2407. PCR with genomic dna - (reply: 9)
2408. mutagenesis - (reply: 1)
2409. Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
2410. PCR with long primers - (reply: 3)
2411. Need Help: Bacterial gene deletion- sacB sucrose resistance - (reply: 31)
2412. Gateway cloning - (reply: 8)
2413. RNA Isolation - RNA isolation from human cell lines (reply: 2)
2414. Which red fluorescence is better? - mCherry of dsRed (reply: 2)
2415. Finding corresponding amino acid in DNA sequence - (reply: 1)
2416. Buffer for 5 Kb DNA fragments - (reply: 2)
2417. Pooling doesn't work - make up rna amount of 10ug (reply: 3)
2418. Western Blot for Bacteria - Loading Control (reply: 1)
2419. uggly, non-focus and diffuse bands in my Western.. - Can you see my image of my Western to LC3? (reply: 1)
2420. Southern blotting - copy number determination (reply: 1)
2421. RNase: RNA ratio - making dsRNA (reply: 1)
2422. I hate my Klenow! - (reply: 9)
2423. Tris Base Buffer is the same that Trizma??No I think.. - Buffers:Differences between Tris Base and Trizma.. (reply: 3)
2424. Sequencing doubts - (reply: 3)
2425. Help with Northern Blotting - (reply: 4)
2426. ELISA - how can i get Mab for NS1? (reply: 1)
2427. 0.5% crystal violet - how to make (reply: 2)
2428. Inversed PCR issues - DNA isolation, restriction, ligation and PCR with no success (reply: 1)
2429. cDNA 260/280 ratio - (reply: 3)
2430. Amino acid substitution a problem? - Phenylalanine to leucine (reply: 3)