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Transcription Related Discussions
  1. "Gene expression vs Transcription vs mRNA levels": Help with conceptua - (reply: 1)
  2. mRNA levels, transcription or Gene expression? - (reply: 2)
  3. Wnt/b-catenin pathway - LEf/TCF transcription activity (reply: 1)
  4. In vitro transcription: why double-banded result? - (reply: 3)
  5. A+G Maxam Gilbert ladder for predicting transcription start site - (reply: 2)
  6. QIAquick PCR purification kit for in vitro transcription? - (reply: 6)
  7. when to set up a RT-PCR for mRNA expression? - detect transcription of transgene (reply: 1)
  8. checking mRNA expression by reverse transcriptase PCR - (reply: 2)
  9. How to improve DNase efficiency after in vitro transcription? - (reply: 3)
  10. Help with DIG UTP Pobe Construction In Vitro Transcription - (reply: 1)
  11. need to identify domain of my sp1 transcription factor - sp1 domain (reply: 1)
  12. spatial and temporal patterns of transcription - (reply: 3)
  13. Invitro Transcription - size distribution of transcripts - (reply: 1)
  14. different transcription from 5' UTR than 3' UTR primers - (reply: 8)
  15. Beta-gal reporter assay = transcription? - How does this work? (reply: 2)
  16. Predicting transcriptional start site (tss) for sigma 70 promoter - (reply: 1)
  17. Question about interpretation of Northern Blots - apparent incomplete transcription of genes in bacteria (reply: 3)
  18. Question on transcription start site and promoter cloning - (reply: 3)
  19. ChIP-Chip Hybridizations - Different method for Histones vs transcription factors? (reply: 1)
  20. Can do reverse transcription by gene-specific primers? - (reply: 3)
  21. Transcription Factors - (reply: 4)
  22. Primer design (long transcripts) - (reply: 2)
  23. One of these days - Is this a critical mistake - reverse transcription (reply: 2)
  24. "read -through" transcription - (reply: 1)
  25. qrt-pcr on bacterial transcripts - (reply: 1)
  26. New construction - for transcription factors (reply: 1)
  27. Thermostable Reverse Transcriptase for RT-PCR - (reply: 3)
  28. Dnase digestion after in vitro transcription - (reply: 4)
  29. Detecting Transcription factors in western blot - Hard time detecting LEF-1(TFs) (reply: 2)
  30. Reverse Transcription, Heated Lid yes or no? - Is it necessary to use heated lid during cDNA synthesis incubations? (reply: 6)
  31. reverse transcription 101 - how to do it from the basics (reply: 2)
  32. Reverse transcription validation and cDNA quantitation - (reply: 1)
  33. The best reverse transcriptase for RT PCR - (reply: 5)
  34. How do transcription factors come together for transcription? - Stochastic process? or Diffusion? When / How is this regulated?!! (reply: 2)
  35. which is more sensitive: IP or western blot? - Looking for transcription factor - would IP help? (reply: 5)
  36. How to identify transcription factors binding to an specific DNA sequence? - (reply: 5)
  37. Transcription factors regulation - testing the effect of NFkB on expression (reply: 2)
  38. DNase Digestion and Reverse Transcription - (reply: 10)
  39. not reproducible : reverse - transcription PCR - (reply: 5)
  40. In vitro Transcription - (reply: 1)
  41. Will ATP inhibit Reverse Transcription? - (reply: 2)
  42. online tool needed for transcriptional factor binding sites prediction - (reply: 1)
  43. RT-PCR: Band in no reverse transcriptase lane - (reply: 18)
  44. confirming equal expression of transcripts in three samples...how to? - (reply: 3)
  45. multiplex reverse transcription - (reply: 2)
  46. transcription of the genes in one operon - (reply: 1)
  47. Inhibition of mRNA transcription - (reply: 5)
  48. Transcription factor assays without radiolabel - Can it be done? (reply: 2)
  49. Reverse Transcriptase PCR - Step by step protocol (reply: 6)
  50. About Reverse transcriptase PCR followed by PCR - primers (reply: 1)
  51. How do you find all the genes regulated by a particular transcription factor? - (reply: 1)
  52. how to see low kd protein in vitro transcription/translation? - (reply: 4)
  53. reverse transcription from viral rna - (reply: 9)
  54. Can transcriptional regulation research be done in vivo? - No cell line is availbale (reply: 7)
  55. HELP! In-vitro transcription results only in smear! - (reply: 2)
  56. in vitro transcription - transcription of long transcripts (reply: 1)
  57. pri-miRNA transcript - get the exact primary transcripts of miRNAs (reply: 4)
  58. Transcription factors studies - (reply: 2)
  59. primer for real-time and reverse transcriptase PCR - (reply: 1)
  60. reverse transcription PCR - (reply: 1)
  61. can transcription happen in cytoplasm? - This question had been posted in the General Biology Discussion board (reply: 6)
  62. transcription factors - (reply: 2)
  63. transcription in prokaryotes. - is whole shine-dalgarno sequence required? (reply: 1)
  64. pcr product afetr reverse transcriptase reaction - 4 bands i have not included the band which i want??? (reply: 5)
  65. can microarray figure out the the downstream target of a putative transcription - (reply: 2)
  66. A successful reverse transcription reaction - (reply: 1)
  67. Western Detection of Transcription Factors - -more difficult than other proteins? (reply: 3)
  68. Reverse Transcription RT-PCR - Help: which machines to use? (reply: 1)
  69. Does transcription factor affect mRNA half-life? - (reply: 7)
  70. ChIP control for transcriptional repressor? - (reply: 1)
  71. PCR after in vitro transcription and translation - (reply: 2)
  72. Which in vitro transcription kit is better? - (reply: 3)
  73. in vitro transcription with T7 promoter - (reply: 3)
  74. what's the sufficient concentration of RNA for reverse transcription? - (reply: 7)
  75. Question on Transcriptional Fusion lacZ - Question on Protocol (reply: 3)
  76. transcription factor search - (reply: 2)
  77. database for transcription factor binding sites - (reply: 1)
  78. Whether Mg2+ can be released from the Reverse Transcriptase after Its Inactivati - Urgent (reply: 3)
  79. Sense or antisense? How can I tell directionality of insert? - Trying to make an antisense riboprobe via in vitro transcription with DIG-UTP fo (reply: 1)
  80. How to check if reverse transcription went OK and there is cDNA - (reply: 5)
  81. Increased transcripts after RNAi - (reply: 10)
  82. What makes a gene a transcription Factor - (reply: 4)
  83. Reverse Transcription with oligo dT or hexamer primers? - What do you prefer and why? (reply: 3)
  84. prediction of transcription factor binding sites - which strand predictions are more important (reply: 1)
  85. choice of reverse transcriptase - (reply: 4)
  86. In vitro transcription/translation with pDest14 - (reply: 1)
  87. Database/Search for Transcription Factor binding sites in promotor regions - (reply: 2)
  88. Reverse Transcriptase PCR - why radiolabeling? (reply: 4)
  89. How to find transcription start site? - (reply: 2)
  90. Transcription start site determination - (reply: 2)
  91. Reverse Transcription question - ssproduct or dsproduct (reply: 3)
  92. cloning/amplifying attenuate RNA transcripts - (reply: 6)
  93. preferential amplification among transcripts - (reply: 1)
  94. Technology for gene knockout at transcriptional level - (reply: 3)
  95. Transcriptional question! - (reply: 4)
  96. Reverse transcription of poly-A tailed miRNAs using anchored Oligo dT primers?&# - (reply: 6)
  97. Consensus transcription factor binding sites - how are the bases denoted (reply: 6)
  98. Getting rid of DNA template from in vitro transcription rxn - (reply: 6)
  99. Reverse transcription - (reply: 1)
  100. general inhibition of transcription - ways to check inhibtion of transcription (reply: 4)
  101. Problem in purification of a bacterial transcription factor - Help...please....I only have 5 months left (reply: 4)
  102. Urea-PAGE for transcription assays - Diffusion of low molecular weight bands (reply: 2)
  103. Question about reverse transcription of bacteria mRNA - (reply: 3)
  104. Does Reverse Transcription have to be done using PCR - (reply: 6)
  105. Reverse transcriptase PCR - RTPCT (reply: 2)
  106. Transcriptional factor domains - (reply: 3)
  107. RNA preparation from in vitro transcription - (reply: 6)
  108. problem with reverse transcription - (reply: 3)
  109. Does Taq have reverse transcriptase activity? - (reply: 20)
  110. Help! RNA in vitro transcription - (reply: 3)
  111. Promoter analysis for transcription factors - (reply: 1)
  112. transcription factor - (reply: 3)
  113. Reverse transcription - (reply: 2)
  114. how to find transcription start site? - (reply: 2)
  115. Reverse Transcription Kits - trying to decide which one to use (reply: 1)
  116. Plasmid template preparation for in vitro transcription - (reply: 9)
  117. transcription - (reply: 1)
  118. Which temperature in Reverse Transcription? - (reply: 4)
  119. Reverse transcription problems - (reply: 8)
  120. transcription factor ChIP - High signal in no Ab control (reply: 5)
  121. Ion-exchange chromatography - Purification of a transcriptional regulator (reply: 3)
  122. SOS! how to search all the possible transcription binding site - (reply: 3)
  123. Reverse Transcription - (reply: 2)
  124. concentration of cDNA after reverse transcription? - (reply: 5)
  125. in-vitro transcription - (reply: 4)
  126. how to find transcription factor binding sites in a sequence? - (reply: 4)
  127. transcription factors - (reply: 2)
  128. Transcription factor database - (reply: 6)
  129. cDNA synthesis - which transcriptase is better? - (reply: 2)
  130. How to transfect siRNA into the nucleus for transcriptional silencing? - Which transfection reagent? (reply: 9)
  131. Why is DTT used in reverse transcription (RT) reaction? - (reply: 9)
  132. SNP locus - SNP distance (bp) to major transcription initiatio (reply: 3)
  133. ChIP against transcription factors - (reply: 7)
  134. How to find the transcription start site of an unknown gene? - (reply: 1)
  135. Transcription Rate - Measure Hn RNA using Taqman?? (reply: 1)
  136. Transcriptional activity in Stable cell line. - (reply: 3)
  137. RT-PCR, why two PCR steps? - two PCRs after reverse transcription?? (reply: 2)
  138. problem with TNT coupled invitro transcription/ - (reply: 1)
  139. Myb transcription factors - question (reply: 1)
  140. transcription inhibition withactinomycinD - (reply: 1)
  141. determining incorrectly spliced transcripts - (reply: 4)
  142. invitro transcription - (reply: 1)
  143. Questions about transcription factors - (reply: 1)