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Consensus transcription factor binding sites - how are the bases denoted (Jan/24/2006 )

I am analysing TF binding sites

In a 1997 paper the consensus
GDRRADYCCC and
In a 2004 paper the following consensus
GGGRNNYYCC

is said to be the consensus for NF kappa beta

I want to know
1) Why these consensus are different.. I am thinking maybe because it changed as new findings were published..am I right?
2) What do each of those letters mean ?
like D, R, N,Y

please let me know of these if you know

thanks

-Watson-

d = ?

N = any base

Y = pyrimidine

R = purine

I think that NF-kB changes with time and there are many subunits involved; perhaps a given consensus alters with new findings, but I think there is some flexibility as well

-aimikins-

is there a website where I can analyse my sequence for NF-kappa-B sites based on this consensus or is that something to be done manually

-Watson-

www.genomatix.de

use matinspector

it will give them all to you if you are patient, with probabilities too...and this part is free. with a little elbow grease you can get some very good potential sites with only the free stuff.

I used it prior to designing some emsa oligos smile.gif

-aimikins-

You might also try the San Diego Supercomputer

http://workbench.sdsc.edu

This has lots of extremely useful nucleic acid analysis tools and a very speedy sequence search function

-Doc_Martin-

TFSearch is also a useful web based search for transcription factors for your DNA sequence of interest.
Ceri

-Ceri-

QUOTE (aimikins @ Jan 24 2006, 09:54 PM)
d = ?

N = any base

Y = pyrimidine

R = purine

I think that NF-kB changes with time and there are many subunits involved; perhaps a given consensus alters with new findings, but I think there is some flexibility as well



D= A or G or T

here is the genetic code : http://www.neb.com/nebecomm/tech_reference...enetic_code.asp

-Missele-