Technology for gene knockout at transcriptional level - (Feb/01/2006 )
Has this kind of technology:
The technology can make any gene in chromosomes don't transcribing at the DNA level.
Because RNAi usually makes gene express down, and sometimes interferes homologous genes expressing.
But this technology didn't have this problem.
Do you mean silencing gene at transcriptional level? It is possible. You can achieve it by targeting some particular promoter sequence using dsRNA.
yes,you said is right.
But sometimes dsRNA usually make transcriptional level down,not complete silencing.
so ,Has this kind of technology? It can make any genetic code in targeting gene mutate whatever you want.
For example: TAC->TAA
This mutationg can complete silence the gene at transcriptional level.
So that is knockout. The following two references use adeno-associated virus vectors to knockout gene expression in cultured cells.
Hirata R, Chamberlain J, Dong R, Russell DW.
Targeted transgene insertion into human chromosomes by adeno-associated virus vectors.
Nat Biotechnol. 2002 Jul;20(7):735-8.
Kohli M, Rago C, Lengauer C, Kinzler KW, Vogelstein B.
Facile methods for generating human somatic cell gene knockouts using recombinant adeno-associated viruses.
Nucleic Acids Res. 2004 Jan 2;32(1):e3.