High initial fluorescence - need help with some results... (Oct/18/2007 )

Hi!! Can you help me with this?

This is the situation:

- a particular cDNA, 2 10-fold dilution (1 and 2)
- dilution 2 shows initial fluorescence (Rn) at 1...
- dilution 1 shows initial fluorescence (Rn) at 3!!

So when the program (MxPro) plots the fluorescence as dRn, both dilution show similar Cts... = BAD RESULT
This only happen with some of the cDNAs tested, other cDNAs (I mean another samples) on the same plate work fine...

What does this high initial fluorescence mean?? I dont think its due to oversaturation right? 'cause once I used too concentrated cDNAs and only the Ct appeared earlier, the initial fluorescence was the same (or approximately) for every dilution.

Thanks for the help!!

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first, is it repeatable for those samples?

second, are those particular wells in your machine contaminated with fluoresence?

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Yes, it is repeatable for those particular samples... and no, it doesnt appear to be any contaminated well in the machine...

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