My PCR product is 100bp (approx). What result is more reliable-gel or hybridisat - (Sep/27/2006 )

Hi,
What is the more reliable result to check for a 100bp PCR product.
A 6% gel or a hybridisation on membrane.

Balaji

6% polyacrylamide?

What's wrong with a standard agarose gel? A 1% gel should do fine. Just run it at 100 V for 45 minutes (a little less than usual) and with a 50 bp ladder.

Or a 3.5% agarose, if one has to be extra careful and count the difference in 10s of bps.

Yeah, I guess it depends how clean you PCR is.

Hello,

If you know the sequence of the 100bp product than hyridization would clearly provide better specificity.


Hi,
What is the more reliable result to check for a 100bp PCR product.
A 6% gel or a hybridisation on membrane.

Balaji
[/quote]

just use a 1 or 2% agarose gel, it should b fine.