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61. Removal of cleaved peptide after Protease cleavage - (reply: 4)
62. Bradford positive but no Protein on SDS PAGE - (reply: 4)
63. Disadvantage of crossflow filtration - (reply: 3)
64. Help with Superdex200 increase Column - (reply: 1)
65. Thrombin cleaved GST-fusion protein- multiple bands - (reply: 2)
66. Loss of protein expression by transformed bacteria - (reply: 4)
67. Cloning several metabolic enzymes to screen for inhibition, how long? - (reply: 1)
68. after protein purification - (reply: 4)
69. Tryple express vs scraping - (reply: 2)
70. Pierce NE-PER final insoluble pellet, is it chromatin and how to solubilize it? - (reply: 1)
71. Predicting amount maximum of sample to apply onto cation exchange chromatography - (reply: 5)
72. recombinant protein vs native protein - (reply: 1)
73. protein expression site - (reply: 3)
74. Beads pull down only free GST - (reply: 14)
75. Where to obtain strain E. coli K12 SB536 ? - (reply: 3)
76. Protein Expresion and Isolation from Lactobacillus - Insoluble protein - (reply: 1)
77. protein expression - (reply: 2)
78. Anion Exchange Buffers - (reply: 2)
79. Difficulty eluting His-tag Zn metalloprotease - (reply: 1)
80. about sds-page buffers - (reply: 1)
81. How do I extract soluble protein after adding SDS-PAGE buffer? - (reply: 1)
82. Best epitope for IF with a poor-expresser - (reply: 2)
83. Collagen binding protein expression E. coli His-tag - (reply: 1)
84. pet-32 - (reply: 3)
85. Elute fractions that have target protein in FPLC is unstable - (reply: 8)
86. How can i enrich highly -ve charge biomolecules using High Q resin? - (reply: 1)
87. Question about MBP purification - (reply: 2)
88. unexpected protein bands - (reply: 2)
89. Urea effect on mice spleen cells - (reply: 1)
90. Non-pH-sensitive fluorophore for relative protein quantification - (reply: 1)
62. Bradford positive but no Protein on SDS PAGE - (reply: 4)
63. Disadvantage of crossflow filtration - (reply: 3)
64. Help with Superdex200 increase Column - (reply: 1)
65. Thrombin cleaved GST-fusion protein- multiple bands - (reply: 2)
66. Loss of protein expression by transformed bacteria - (reply: 4)
67. Cloning several metabolic enzymes to screen for inhibition, how long? - (reply: 1)
68. after protein purification - (reply: 4)
69. Tryple express vs scraping - (reply: 2)
70. Pierce NE-PER final insoluble pellet, is it chromatin and how to solubilize it? - (reply: 1)
71. Predicting amount maximum of sample to apply onto cation exchange chromatography - (reply: 5)
72. recombinant protein vs native protein - (reply: 1)
73. protein expression site - (reply: 3)
74. Beads pull down only free GST - (reply: 14)
75. Where to obtain strain E. coli K12 SB536 ? - (reply: 3)
76. Protein Expresion and Isolation from Lactobacillus - Insoluble protein - (reply: 1)
77. protein expression - (reply: 2)
78. Anion Exchange Buffers - (reply: 2)
79. Difficulty eluting His-tag Zn metalloprotease - (reply: 1)
80. about sds-page buffers - (reply: 1)
81. How do I extract soluble protein after adding SDS-PAGE buffer? - (reply: 1)
82. Best epitope for IF with a poor-expresser - (reply: 2)
83. Collagen binding protein expression E. coli His-tag - (reply: 1)
84. pet-32 - (reply: 3)
85. Elute fractions that have target protein in FPLC is unstable - (reply: 8)
86. How can i enrich highly -ve charge biomolecules using High Q resin? - (reply: 1)
87. Question about MBP purification - (reply: 2)
88. unexpected protein bands - (reply: 2)
89. Urea effect on mice spleen cells - (reply: 1)
90. Non-pH-sensitive fluorophore for relative protein quantification - (reply: 1)