|
421. strep-tag II purification not pure - how to make it pure with one step purification or rather do two step (reply: 3)
422. How to be good at prep? - Calling all protein experts (reply: 2)
423. Protein Migration Problem [S.O.S.] - Moves in SDS-PAGE but not in Native (reply: 8)
424. His-tagged protein purification - (reply: 3)
425. protein purification - (reply: 3)
426. pGEX vectors and GST expression - (reply: 3)
427. contamination in protein expression - I got a couple of questions (reply: 3)
428. Always expressing truncated protein - (reply: 3)
429. Over Experssion of A Protein - (reply: 3)
430. IPTG induction not observable or noticeable - (reply: 2)
431. Problem with protein purification By FPLC - (reply: 4)
432. Protein precipitation with TCA? - Protocols, suggestions, ideas, experiences (reply: 2)
433. His-tag proteins not eluted from Ni-Beads - (reply: 2)
434. Expressing a Gene or an ORF in an Expression Vector? - (reply: 4)
435. Recombinant Antibody Production -Reducing / Non-reducing SDS page conditions - (reply: 2)
436. trouble with protein expression of high AT rich gene - (reply: 1)
437. Preparation of Purified Inclusion Bodies - (reply: 2)
438. Denaturing IB in 8 M Urea - (reply: 3)
439. Promega IVT Problems - Low expression (reply: 1)
440. weak bands - western blots (reply: 1)
441. problem in M9 media - m9 media (reply: 4)
442. Help for a purificaltion newbie - (reply: 2)
443. Immunioprecipitation from Jurkat cells?? - (reply: 1)
444. Problems with Soluble and Insoluble Protein Fraction - (reply: 4)
445. antibody HRP - (reply: 2)
446. Western blot transfer weak - (reply: 1)
447. Home-made Size-exclusion Columns? - (reply: 1)
448. how to get pure protein - (reply: 1)
449. co-Immunoprecipitation control question - co-IP (reply: 1)
450. to get protein in cytosol from inclusion bodies - (reply: 4)
422. How to be good at prep? - Calling all protein experts (reply: 2)
423. Protein Migration Problem [S.O.S.] - Moves in SDS-PAGE but not in Native (reply: 8)
424. His-tagged protein purification - (reply: 3)
425. protein purification - (reply: 3)
426. pGEX vectors and GST expression - (reply: 3)
427. contamination in protein expression - I got a couple of questions (reply: 3)
428. Always expressing truncated protein - (reply: 3)
429. Over Experssion of A Protein - (reply: 3)
430. IPTG induction not observable or noticeable - (reply: 2)
431. Problem with protein purification By FPLC - (reply: 4)
432. Protein precipitation with TCA? - Protocols, suggestions, ideas, experiences (reply: 2)
433. His-tag proteins not eluted from Ni-Beads - (reply: 2)
434. Expressing a Gene or an ORF in an Expression Vector? - (reply: 4)
435. Recombinant Antibody Production -Reducing / Non-reducing SDS page conditions - (reply: 2)
436. trouble with protein expression of high AT rich gene - (reply: 1)
437. Preparation of Purified Inclusion Bodies - (reply: 2)
438. Denaturing IB in 8 M Urea - (reply: 3)
439. Promega IVT Problems - Low expression (reply: 1)
440. weak bands - western blots (reply: 1)
441. problem in M9 media - m9 media (reply: 4)
442. Help for a purificaltion newbie - (reply: 2)
443. Immunioprecipitation from Jurkat cells?? - (reply: 1)
444. Problems with Soluble and Insoluble Protein Fraction - (reply: 4)
445. antibody HRP - (reply: 2)
446. Western blot transfer weak - (reply: 1)
447. Home-made Size-exclusion Columns? - (reply: 1)
448. how to get pure protein - (reply: 1)
449. co-Immunoprecipitation control question - co-IP (reply: 1)
450. to get protein in cytosol from inclusion bodies - (reply: 4)