How do I extract soluble protein after adding SDS-PAGE buffer? - (Jan/26/2015 )

Hi everyone,

I had a bit of a mess up in the lab today. I'm currently trying to overexpress a protein in E.coli, and I'm analysing the samples from the pilot expression. So basically I mixed up my 'induced' tubes with my 'non-induced' tubes when preparing my samples to run on an SDS-page gel, so my 'induced' cells have been resuspended in SDS-PAGE sample buffer and boiled without separation of soluble and insoluble protein. I've tried looking online but can't for the life of me figure out what I have to do to separate them now. Any ideas? 

Thanks!

Not much you can do now.

If the protein expresses really well, just do the same to the un-induced cells and you could see a large band corresponding to your target protein in you induced but not uninduced cells.

Or just start over. Day's work lost...no big deal.