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Protein-Expression-and-Purification
151.
Protein Purification-catastrophe while purifying? -
(reply: 2)
152.
T5 Promoter -
(reply: 1)
153.
Protein purification for adherent mammalian cells -
(reply: 2)
154.
Antibody production in Expi293 cells -
(reply: 2)
155.
pGEX-2t purification problem -
(reply: 3)
156.
Phosphorylation of recombinant proteins in E. coli -
(reply: 1)
157.
Compatibility of Urea and Imidazole -
(reply: 2)
158.
Growth media - OD at induction - growth phases... -
(reply: 2)
159.
NusA tag produces protein truncations? -
(reply: 3)
160.
Tissue-Specific Protein Expression Database -
(reply: 4)
161.
Problem with strep tagged protein purification -
(reply: 5)
162.
why i get double band for recombinant protein -
(reply: 9)
163.
Pulldown with nickel beads? -
(reply: 3)
164.
protein purification in acanthamoeba lysate -
(reply: 3)
165.
GPCR expression in HEK293 -
(reply: 2)
166.
screening Protein Expression -
(reply: 1)
167.
Polyclonal Ab purification -
(reply: 1)
168.
GST tag fusion protein--- help please -
(reply: 4)
169.
purify protein from native gel -
(reply: 1)
170.
purifying proteins with repeats -
(reply: 3)
171.
chromatography question -
(reply: 2)
172.
Protein purification from greenish supernatant of Pichia pastoris -
(reply: 10)
173.
Streptavidin purification in the lab -
(reply: 4)
174.
Membrane protein -
(reply: 1)
175.
adding Tween80 to bacterial culture for protein purification ?! -
(reply: 3)
176.
1M IPTG left for 12-14h @ Room temp - still good? -
(reply: 2)
177.
pGEX-KG Vector insert in BL21 Codon Plus RP, Growth Difficulty -
(reply: 4)
178.
Difficulty in Expression of Protein Having 73% GC contents in GST Tag Vectrors -
(reply: 3)
179.
Desalting resin -
(reply: 1)
180.
RNA extraction by trizol -
(reply: 3)
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