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1. Resuspending protein mixture after over-centrifuging - (reply: 1)
2. Virus vs protein purification - (reply: 2)
3. How to eliminate inherent proteins contamination during protein purification? - (reply: 1)
4. A single point mutation enhanced protein expression but attenuated its function - (reply: 4)
5. Why is my His-SUMO tag being cleaved during purification? - (reply: 1)
6. How to obtain a non-cellular protein in its natural form? - (reply: 4)
7. Protein expression issue - (reply: 4)
8. GLUT1 and GLUT4 expression in serum - (reply: 2)
9. Time period of sodiun lauryl sarcosine - (reply: 1)
10. Outer membrane protein extraction - (reply: 5)
11. Virus vs protein purification - (reply: 3)
12. S. aureus lysis protocol for LC-MS/MS - (reply: 2)
13. How to make antibody based affinity column for protein purification - (reply: 4)
14. Agarose beads IP troubles - (reply: 7)
15. Problem with Rosetta gami B protein expression!!!! - (reply: 1)
16. bacterial surface protein extraction - (reply: 1)
17. peptide concentation clearification - (reply: 1)
18. peptide size either in Da or kDa - (reply: 1)
19. determination of protein concentation - (reply: 1)
20. Protein concentration in mM - (reply: 3)
21. polb cloned in pET not expressed - (reply: 2)
22. protein purification from agarose beads using immunoprecipation kit - (reply: 2)
23. Fab antibody fragment purification - (reply: 2)
24. Arctic Express Cpn60 Antibody - (reply: 1)
25. AKTA FPLC - UV instability - (reply: 4)
26. Re-purification of His-tagged protein over IMAC - (reply: 2)
27. Anti-Penta His antibody detecting extra bands - (reply: 2)
28. Pros and Cons of refolding proteins - (reply: 3)
29. pKD1 based vector for K. lactis expression - (reply: 2)
30. HSP70 contamination after Nickel purification - (reply: 3)
2. Virus vs protein purification - (reply: 2)
3. How to eliminate inherent proteins contamination during protein purification? - (reply: 1)
4. A single point mutation enhanced protein expression but attenuated its function - (reply: 4)
5. Why is my His-SUMO tag being cleaved during purification? - (reply: 1)
6. How to obtain a non-cellular protein in its natural form? - (reply: 4)
7. Protein expression issue - (reply: 4)
8. GLUT1 and GLUT4 expression in serum - (reply: 2)
9. Time period of sodiun lauryl sarcosine - (reply: 1)
10. Outer membrane protein extraction - (reply: 5)
11. Virus vs protein purification - (reply: 3)
12. S. aureus lysis protocol for LC-MS/MS - (reply: 2)
13. How to make antibody based affinity column for protein purification - (reply: 4)
14. Agarose beads IP troubles - (reply: 7)
15. Problem with Rosetta gami B protein expression!!!! - (reply: 1)
16. bacterial surface protein extraction - (reply: 1)
17. peptide concentation clearification - (reply: 1)
18. peptide size either in Da or kDa - (reply: 1)
19. determination of protein concentation - (reply: 1)
20. Protein concentration in mM - (reply: 3)
21. polb cloned in pET not expressed - (reply: 2)
22. protein purification from agarose beads using immunoprecipation kit - (reply: 2)
23. Fab antibody fragment purification - (reply: 2)
24. Arctic Express Cpn60 Antibody - (reply: 1)
25. AKTA FPLC - UV instability - (reply: 4)
26. Re-purification of His-tagged protein over IMAC - (reply: 2)
27. Anti-Penta His antibody detecting extra bands - (reply: 2)
28. Pros and Cons of refolding proteins - (reply: 3)
29. pKD1 based vector for K. lactis expression - (reply: 2)
30. HSP70 contamination after Nickel purification - (reply: 3)