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211. Interferon Alpha - (reply: 1)
212. Can you see GFP after lysis mammalian cells? - (reply: 5)
213. Very small peptide expression in E. coli - (reply: 2)
214. C-terminal GST fusion problem in mammalian cell - (reply: 6)
215. Is anti-His antibody detergent sensitive? - (reply: 6)
216. tagged protein size - (reply: 10)
217. Protein expression in e.coli - (reply: 5)
218. Problems with overnights - (reply: 5)
219. problem in extracting the protein - (reply: 1)
220. Protein purification problems: Presence of contaminated proteins along with the - (reply: 5)
221. Protein Purification from Polyacrylamide Gels - (reply: 3)
222. Protein Expression vector with small tag - (reply: 1)
223. Protein precipitated while kept at 4C over night after elution from a Ni-NTA col - (reply: 4)
224. Albumin Removal From BSA - (reply: 1)
225. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
226. Insulin production - (reply: 2)
227. High Yield Protein Expression 10g/L - (reply: 5)
228. No activity of expressed protein obtained from pET32a cloned gene - (reply: 6)
229. GST- tagged protein expressed in insoluble - (reply: 5)
230. Scaling up reagents for large scale immunoprecipitation - (reply: 1)
231. Prepare protein standards for concentration determination - (reply: 1)
232. Inclusion body formation of the recombinant protein expressed in pET28a in E.col - (reply: 3)
233. Tagged recombinant protein has different mobility? - (reply: 7)
234. My protein doesn't bind to the Ni-NTA column - (reply: 11)
235. pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 30)
236. TCA IgG precipitation - (reply: 2)
237. Does triple labeled protein precipitate more easily? - (reply: 2)
238. High gravity crush beads ? - (reply: 1)
239. Peptide recuperation after FactorXa cleavage using GST fusion - (reply: 1)
240. how do GST-tags affect gel filtration results? - (reply: 5)
212. Can you see GFP after lysis mammalian cells? - (reply: 5)
213. Very small peptide expression in E. coli - (reply: 2)
214. C-terminal GST fusion problem in mammalian cell - (reply: 6)
215. Is anti-His antibody detergent sensitive? - (reply: 6)
216. tagged protein size - (reply: 10)
217. Protein expression in e.coli - (reply: 5)
218. Problems with overnights - (reply: 5)
219. problem in extracting the protein - (reply: 1)
220. Protein purification problems: Presence of contaminated proteins along with the - (reply: 5)
221. Protein Purification from Polyacrylamide Gels - (reply: 3)
222. Protein Expression vector with small tag - (reply: 1)
223. Protein precipitated while kept at 4C over night after elution from a Ni-NTA col - (reply: 4)
224. Albumin Removal From BSA - (reply: 1)
225. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
226. Insulin production - (reply: 2)
227. High Yield Protein Expression 10g/L - (reply: 5)
228. No activity of expressed protein obtained from pET32a cloned gene - (reply: 6)
229. GST- tagged protein expressed in insoluble - (reply: 5)
230. Scaling up reagents for large scale immunoprecipitation - (reply: 1)
231. Prepare protein standards for concentration determination - (reply: 1)
232. Inclusion body formation of the recombinant protein expressed in pET28a in E.col - (reply: 3)
233. Tagged recombinant protein has different mobility? - (reply: 7)
234. My protein doesn't bind to the Ni-NTA column - (reply: 11)
235. pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 30)
236. TCA IgG precipitation - (reply: 2)
237. Does triple labeled protein precipitate more easily? - (reply: 2)
238. High gravity crush beads ? - (reply: 1)
239. Peptide recuperation after FactorXa cleavage using GST fusion - (reply: 1)
240. how do GST-tags affect gel filtration results? - (reply: 5)