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Protein-Expression-and-Purification
181.
Problems in expression of target protein in E. coli -
(reply: 3)
182.
Membrane protein extraction -
(reply: 2)
183.
Is it possible to use wireless connection to control and monitor FPLC? -
(reply: 3)
184.
EMSA - general question -
(reply: 1)
185.
Understanding immunoprecipitation - where do the beads go? -
(reply: 2)
186.
Soluble protien of GST -
(reply: 2)
187.
trouble in his-tagged protein binding with Ni-NTA agarose -
(reply: 3)
188.
Contaminants in protein purification.....help! -
(reply: 3)
189.
Protein expression and purification - Please help -
(reply: 2)
190.
Protein purifcation without chromatography -
(reply: 6)
191.
Bacterial Media Formulation -
(reply: 2)
192.
tag for protein purification -
(reply: 7)
193.
Protein is not Binding to DE52 column material -
(reply: 2)
194.
expression problem with pBAD33...similar experience? -
(reply: 2)
195.
MBP-Tagged protein degrading after expression -
(reply: 1)
196.
How to prevent albumin masking of urokinase from conditioned media -
(reply: 3)
197.
How to estimate the protein expression level from the SDS_PAGE? -
(reply: 8)
198.
Controls for co-immunoprecipitation -
(reply: 3)
199.
How to improve enzyme activity? -
(reply: 2)
200.
Protein puriification using IMAC: flowthrough very slow -
(reply: 2)
201.
Sediments/debris/pellet in protein lysate? -
(reply: 3)
202.
Cleavage of GST-tag with Factor Xa -
(reply: 1)
203.
Trouble in GST- fusion protein purification -
(reply: 1)
204.
Helppurification of expressed protein -
(reply: 2)
205.
protein expression by changing start codon -
(reply: 1)
206.
Western Blot - Protein Marker Present on Film After Developing -
(reply: 1)
207.
Odd secondary pellet formed after lysis with B-Per or BugBuster -
(reply: 2)
208.
pcDNA overexpression -
(reply: 4)
209.
membrane proteins ultrafiltration -
(reply: 2)
210.
Partial cleavage of HA (Hemagglutinin) tag? -
(reply: 4)
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