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121. DNA cleanup after linearization, before transformation? - (reply: 3)
122. Can I use cellufine sulfate cloumn as a strong cation exchange? - (reply: 2)
123. predict VLP formation - (reply: 1)
124. need help for transfection to synchronized cells - (reply: 4)
125. Pulldown of polyubiquitinated proteins - (reply: 1)
126. membrane protein expression - (reply: 2)
127. purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
128. Sephacryl S-300 didn't enhance the purity of my enzyme - (reply: 3)
129. Will 5mM DTT break existing disulfide bonds? - (reply: 3)
130. Just part of the CDS (like transmembrane domain) or the full-length CDS for prot - (reply: 3)
131. Protein secretion wtih pFuse expression system in 293T cells - (reply: 2)
132. protein extraction from cell nucleus - (reply: 2)
133. his-tag purification - (reply: 9)
134. selection of the media for ion exchange chromatography - (reply: 1)
135. Designing rPlasmid for Expression in E.Coli: Questions From A Newbie - (reply: 1)
136. Lysis buffer for leptin receptor and pSTAT detection - (reply: 1)
137. Does a C-terminal Proline Residue Increase Degradation Risk? - (reply: 2)
138. His-tag and GST-tag - (reply: 1)
139. Rate of protein production with pcDNA 3.3 transfected into HEK293 - (reply: 3)
140. Do protein purification under partially denatured condition - (reply: 1)
141. Discoloration of Nickel affinity resins during 6-his protein purification - (reply: 1)
142. how to remove affinity tag completely - (reply: 14)
143. Agarose bead size in affinity chromatography - (reply: 2)
144. Protein precipitation by storage - (reply: 11)
145. designing fusion protein with Flag tag - (reply: 1)
146. Problems with biotynylated protein binding to monomeric avidin - (reply: 1)
147. Control protein for eukaryotic expression - (reply: 5)
148. Flag fusion protein not detected by Flag antibody - (reply: 8)
149. E. coli culture - (reply: 1)
150. instability index of expressed protein - (reply: 1)
122. Can I use cellufine sulfate cloumn as a strong cation exchange? - (reply: 2)
123. predict VLP formation - (reply: 1)
124. need help for transfection to synchronized cells - (reply: 4)
125. Pulldown of polyubiquitinated proteins - (reply: 1)
126. membrane protein expression - (reply: 2)
127. purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
128. Sephacryl S-300 didn't enhance the purity of my enzyme - (reply: 3)
129. Will 5mM DTT break existing disulfide bonds? - (reply: 3)
130. Just part of the CDS (like transmembrane domain) or the full-length CDS for prot - (reply: 3)
131. Protein secretion wtih pFuse expression system in 293T cells - (reply: 2)
132. protein extraction from cell nucleus - (reply: 2)
133. his-tag purification - (reply: 9)
134. selection of the media for ion exchange chromatography - (reply: 1)
135. Designing rPlasmid for Expression in E.Coli: Questions From A Newbie - (reply: 1)
136. Lysis buffer for leptin receptor and pSTAT detection - (reply: 1)
137. Does a C-terminal Proline Residue Increase Degradation Risk? - (reply: 2)
138. His-tag and GST-tag - (reply: 1)
139. Rate of protein production with pcDNA 3.3 transfected into HEK293 - (reply: 3)
140. Do protein purification under partially denatured condition - (reply: 1)
141. Discoloration of Nickel affinity resins during 6-his protein purification - (reply: 1)
142. how to remove affinity tag completely - (reply: 14)
143. Agarose bead size in affinity chromatography - (reply: 2)
144. Protein precipitation by storage - (reply: 11)
145. designing fusion protein with Flag tag - (reply: 1)
146. Problems with biotynylated protein binding to monomeric avidin - (reply: 1)
147. Control protein for eukaryotic expression - (reply: 5)
148. Flag fusion protein not detected by Flag antibody - (reply: 8)
149. E. coli culture - (reply: 1)
150. instability index of expressed protein - (reply: 1)