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Protein-Expression-and-Purification
91.
Validating Recombinant Protein -
(reply: 1)
92.
Sonication of cells in ultrasound bath, without probe -
(reply: 3)
93.
Proteinase extraction and purification -
(reply: 1)
94.
E.Coli Lysis: To add Lysozyme or not to add Lysozyme, that is the question -
(reply: 2)
95.
EGFP expression -
(reply: 1)
96.
the same construct, two stable cell clones with different protein size -
(reply: 2)
97.
Purification of metallothioneins? -
(reply: 1)
98.
RIPA buffer for IP and western -
(reply: 1)
99.
i am trying to get rid of unexpected protein band by centricon -
(reply: 3)
100.
CO- IP for nuclear protein -
(reply: 4)
101.
Trichloroacetic Acid precipitation of proteins -
(reply: 2)
102.
Problems with a nonspecific interaction on a Ni-NTA column -
(reply: 3)
103.
protein expression with and without induction -
(reply: 1)
104.
Please help me :Protein expression and purification -
(reply: 1)
105.
Troubleshooting: His-Tag Nickle Column Purification -
(reply: 1)
106.
genotype of e coli strain for protein expression -
(reply: 2)
107.
Problems with detection of protein expressed in yeast -
(reply: 1)
108.
thionin -
(reply: 1)
109.
How do I go about making a set of protein standards for protein quantification v -
(reply: 2)
110.
secretome and zymograms -
(reply: 1)
111.
Protein Refolding - Precipitation during dialysis -
(reply: 3)
112.
Using a mouse antibody for IP - should I use Dynabeads with Protein G or with sh -
(reply: 1)
113.
Problem with calculations for enzyme activity -
(reply: 2)
114.
problem with sds-page -
(reply: 8)
115.
Ammonium sulfate fractionalization help -
(reply: 1)
116.
heme protein precipitation -
(reply: 4)
117.
Transient transfection using linear 25 kDa PEI transfection reagent in CHO k1 -
(reply: 4)
118.
regarding protein purification using 8m urea and sds page -
(reply: 2)
119.
how do you inactivate His-tag affinity beads?? -
(reply: 4)
120.
Protein Purification from mammalian cells -
(reply: 1)
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