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Protein-Expression-and-Purification
391.
Expressing human protein in E.Coli -
(reply: 12)
392.
Sample preparation for SDS from detergent insoluble fraction (Pellet). -
(reply: 2)
393.
Help with GST protein purification -
(reply: 4)
394.
Antiobiotics for protein expression -
(reply: 3)
395.
how to extract proteins from HEK293 - I need your experience
(reply: 3)
396.
Identification of purity of the protein -
(reply: 2)
397.
Polysorbate 80 (Tween 80) removal -
(reply: 13)
398.
Maltose Binding Protein Sequence - A sequence for the Maltose Binding Protein.
(reply: 1)
399.
nuclear extraction of blood cells -
(reply: 2)
400.
Is My Protein Pure or Not? - Protein purification, SDS-Page
(reply: 19)
401.
volume to load sample for affinity chromatography -
(reply: 6)
402.
Reporter gene to test new promotor -
(reply: 1)
403.
Protein Phosphorylation -
(reply: 2)
404.
His-Tag protein purification:protein eluted with NaOH??? -
(reply: 3)
405.
His-tag purification post Enterokinase digestion - Buffer exchange?
(reply: 1)
406.
Folding -
(reply: 2)
407.
C-terminal Cleavage During Overexpression -
(reply: 3)
408.
FOR immunoprecipitation in plant , flag tag or His tag -
(reply: 2)
409.
Loss of protein upon concentration -
(reply: 4)
410.
Problems with Bacterial Induction: NO Induction -
(reply: 1)
411.
His-tag protein purification - aggregation of protein
(reply: 5)
412.
After inducing with IPTG for protein expression, my cell pellet after centrifuga -
(reply: 1)
413.
Coimmunoprecipitation using Ni-NTA, My non-his-tagged target bind to beads -
(reply: 3)
414.
Protein Purification of an His-Tag Protein using a Ni Column -
(reply: 1)
415.
Decrease in concentration after protein purification -
(reply: 3)
416.
Ammonium sulphate precipitation Problem - Precipitants couldnt be seen on SDS-PAGE and WB
(reply: 9)
417.
Bacterial Induction: Same concentration of protein samples? -
(reply: 1)
418.
ways to stop soluble aggregation -
(reply: 9)
419.
need protocol on purfication of allinase from raw garlic -
(reply: 2)
420.
labeling a protein -
(reply: 3)
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