Urea effect on mice spleen cells - (Nov/23/2014 )

Hi,

I am going to do lymphocyte proliferation assay using mouse spleen cells in order to assess the cytokine profile of the cells (IFN-gamma, IL-2 and IL-10) after induction with the target protein. I have purified my recombinant protein of interest using denaturing method by 6M Urea. Since my protein disappears after dialysis, I wondered if I could use the protein for inducing cells without dialysis. I would like to know if urea has any negative effect on spleen cells' growth?

Thanks,

no need to double post