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271. brain tissue homogenization with sample buffer?? - (reply: 2)
272. Low expression levels of highly soluble protein - why is ON expression not helpi - (reply: 8)
273. Reversed-Phase chromatography for protein purification - (reply: 3)
274. Nickel IMAC for His-tag - column goes dark brown! What does this mean? - (reply: 6)
275. rare codon usage in e coli expression very urgent!!!!!!& - (reply: 4)
276. Easy method to lyse E. coli pellet (follow-up of GST fusion protein purification - (reply: 4)
277. need help with gst-tagged protein-purification - (reply: 1)
278. Active enzyme goes on ion exchange column -- dead enzyme comes off. What am I do - (reply: 3)
279. Protein degradation during expression - (reply: 3)
280. Diff. between S75 16/60 and S200 16/60? - (reply: 1)
281. Expression of insoluble protein - (reply: 4)
282. Weak affinity chromatography - (reply: 2)
283. NL and L? - (reply: 2)
284. e coli vector and host selection - (reply: 1)
285. Double tagging - (reply: 1)
286. Purifying recombinant protein from pellet? - (reply: 2)
287. Co-expression of 2 plasmids in E.coli - (reply: 1)
288. Protein ladder - (reply: 1)
289. Trouble producing stable cell lines - (reply: 2)
290. Can anyone help me to identify what line is that??? - (reply: 4)
291. enrichment with wheat germ agglutinin (WGA) - (reply: 1)
292. membrane protein expression - (reply: 1)
293. Magnesium acetate in protein lysates - (reply: 1)
294. Protease Inhibitor Cocktails - (reply: 3)
295. E. coli cells refuse to grow! - (reply: 2)
296. Extra bands after protein expression/purification - (reply: 4)
297. Better protein Expression? - (reply: 2)
298. Expression of protein with pI 8.2 - (reply: 1)
299. Methionine for protein expression - (reply: 3)
300. Alternatives to benzonase? - (reply: 1)
272. Low expression levels of highly soluble protein - why is ON expression not helpi - (reply: 8)
273. Reversed-Phase chromatography for protein purification - (reply: 3)
274. Nickel IMAC for His-tag - column goes dark brown! What does this mean? - (reply: 6)
275. rare codon usage in e coli expression very urgent!!!!!!& - (reply: 4)
276. Easy method to lyse E. coli pellet (follow-up of GST fusion protein purification - (reply: 4)
277. need help with gst-tagged protein-purification - (reply: 1)
278. Active enzyme goes on ion exchange column -- dead enzyme comes off. What am I do - (reply: 3)
279. Protein degradation during expression - (reply: 3)
280. Diff. between S75 16/60 and S200 16/60? - (reply: 1)
281. Expression of insoluble protein - (reply: 4)
282. Weak affinity chromatography - (reply: 2)
283. NL and L? - (reply: 2)
284. e coli vector and host selection - (reply: 1)
285. Double tagging - (reply: 1)
286. Purifying recombinant protein from pellet? - (reply: 2)
287. Co-expression of 2 plasmids in E.coli - (reply: 1)
288. Protein ladder - (reply: 1)
289. Trouble producing stable cell lines - (reply: 2)
290. Can anyone help me to identify what line is that??? - (reply: 4)
291. enrichment with wheat germ agglutinin (WGA) - (reply: 1)
292. membrane protein expression - (reply: 1)
293. Magnesium acetate in protein lysates - (reply: 1)
294. Protease Inhibitor Cocktails - (reply: 3)
295. E. coli cells refuse to grow! - (reply: 2)
296. Extra bands after protein expression/purification - (reply: 4)
297. Better protein Expression? - (reply: 2)
298. Expression of protein with pI 8.2 - (reply: 1)
299. Methionine for protein expression - (reply: 3)
300. Alternatives to benzonase? - (reply: 1)